通络保肾复方对LPS刺激的肾小球系膜细胞表达TGF-β、FN、LN的影响  

Influences of Tongluobaoshen Compound Formula on expressions of TGF-β,fibronectin and laminin in GMC induced by LPS

作　　者：宇汝翠[1] 张雁[2] 李平[1] 张梅[1] 陆智慧[1] 温跃才[1] 

机构地区：[1]安徽省立医院,安徽230001 [2]安庆市立医院

出　　处：《北京中医药大学学报》2010年第7期486-488,F0003,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的探讨通络保肾复方对肾小球系膜细胞(GMCs)表达转化生长因子-β(TGF-β)、纤维连接蛋白(FN)、层粘连蛋白(LN)的影响。方法通络保肾复方灌胃大鼠后,制备药物血清,将5%含药血清培养基加入体外培养的GMCs,脂多糖(LPS)作为刺激因子,将细胞分为空白组、LPS组、缬沙坦组及通络保肾复方小、中、大剂量组,刺激GMCs48h后,应用细胞免疫组化方法检测各组GMCs表达TGF-β、FN、LN的情况。结果通络保肾复方能够抑制GMCs表达TGF-β、FN、LN,其中以通络保肾复方大剂量组和中剂量组效果显著,与LPS组比较差异显著(P<0.05)。结论通络保肾复方可以抑制LPS诱导的GMCs表达TGF-β、FN、LN,从而延缓肾小球硬化的进程。Objective To investigate the influences of Tongluobaoshen Compound Formula（TCF） on expressions of TGF-β,fibronectin（FN） and laminin（LN） in the glomerular mesangial cells（GMCs） induced by lipopolysaccharide（LPS） .Method The medicinal serum was prepared after TCF was given to rats orally.The media of 5%medicinal serum was added into GMCs cultured in vitro.LPS was taken as stimulate factor.GMCs were divided into the blank group,LPS group,valsartan group,and low-dose,mid-dose,and high-dose TCF groups.GMCs were stimulated with LPS for 48 hours.The expressions of TGF-β,FN and LN in GMCs in all groups were detected by using cellular immunohistochemistry assay. Result TCF inhibited the expressions of TGF-β,FN and LN,and the effects were more significant in the high-dose and mid-dose TCF groups.The comparison between TCF groups and LPS group showed a significant difference（P0.05） .Conclusion TCF can inhibit the expressions of TGF-β,FN and LN in GMCs induced by LPS,so that glomerular sclerosis was delayed.

关 键 词：通络保肾复方 脂多糖 转化生长因子-β 纤维连接蛋白 层粘连蛋白 肾小球系膜细胞 肾小球硬化 

分 类 号：R285.5[医药卫生—中药学]