替米沙坦对db/db小鼠胰岛内微血管结构的影响  

The effects of telmisartan on intra-islet microvasculature in db/db mice

作　　者：邵加庆[1] 顾萍[1] 杜宏[1] 卢斌[1] 李洁[1] 彭丽[1] 王燕燕[1] 赵明[1] 王坚[1] 

机构地区：[1]南京军区南京总医院内分泌科,210002

出　　处：《中华老年心脑血管病杂志》2010年第8期726-728,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(30900697);江苏省自然科学基金(BK2007574);江苏省"六大人才高峰"资助计划[(2008)101]

摘　　要：目的探讨替米沙坦阻断肾素血管紧张素系统对db/db小鼠胰岛内微血管结构的影响。方法将22只8周龄db/db小鼠随机分为2组:替米沙坦组11只和安慰剂组11只;另选11只同周龄db/m小鼠为非糖尿病组。每周监测小鼠体重、血糖,6周后行腹腔葡萄糖耐量试验(IPGTT)并取胰腺行免疫组织化学检测,分析胰岛内微血管中CD31表达情况。结果治疗6周后,与安慰剂组比较,替米沙坦组小鼠空腹血糖明显降低,胰岛素敏感指数明显升高,差异有统计学意义(P<0.05);非糖尿病组小鼠的体重和空腹血糖明显降低,差异有统计学意义(P<0.05)。替米沙坦组小鼠葡萄糖曲线下面积明显下降,0～30 min胰岛素曲线下面积明显上升,差异有统计学意义(P<0.05);替米沙坦组小鼠胰岛中血管内皮细胞CD31染色强度明显增强,血管数量明显增加。结论替米沙坦阻断血管紧张素Ⅱ1型受体能够改善胰岛微环境,增加胰岛内微血管数量,促进早期相胰岛素分泌,从而保护胰岛β细胞。Objective To explore the effects of telmisartan on intraislet microvasculature in db/db mice through blocking reninangiotensin system.Methods Twenty-two 8 weeks old db/db mice were randomized to telmisartan group（5 mg/kg telmisartan,n=11） and placebo group（n=11）,the drugs were given via gavage for 6 weeks.Other 11 agematched db/m mice were concurrently treated with placebo as nondiabetic controls.Body weight and blood glucose were measured every week.After 6 weeks’ treatment,intraperitoneal glucose tolerance test was performed,immunohistochemical examination of pancreas was carried out to analyze expression of CD31 in microvessels of islet.Results After six weeks’ telmisartan treatment,the fasting blood glucose significantly decreased,insulin sensitivity index significantly increased（P〈0.05）.AUC of glucose curve decreased（P〈0.05） and AUC of 0-30 min insulin curve increased（P〈0.05） in telmisartan group compared with placebo group.Telmisartan treatment greatly increased staining intensity of CD31 in islets.Conclusions Telmisartan treatment remarkably increased intraislet microvasculature volume,and improved earlyphase insulin secretion,thus protected βcell function.

关 键 词：糖尿病 胰岛 肾素-血管紧张素系统 葡糖耐量试验 血糖 安慰剂 

分 类 号：R965[医药卫生—药理学]