猪口蹄疫和水泡病病毒免疫鉴别诊断方法的比较研究  

Comparison of Immunological Detections of Foot and Mouth Disease Virus (FMDV)and Swine Vesicular Disease Virus(SVDV)

作　　者：何永强[1] 杜青云[1] 盛祖恬[1] 孙理云[1] 何秉耀[1] 

机构地区：[1]浙江省农业科学院病毒室

出　　处：《中国预防兽医学报》1999年第3期228-230,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：浙江省科委"八五"攻关课题

摘　　要：通过单克隆抗体斑点酶联免疫吸附试验（ＭｃＡｂＤｏｔＥＬＩＳＡ）和反向间接血凝试验（ＲＩＨＡ）分别对猪口蹄疫病毒（ＦＭＤＶ）和水泡病病毒（ＳＶＤＶ）检测比较。试验表明，ＭｃＡｂＤｏｔＥＬＩＳＡ特异性强，快速简便，比ＲＩＨＡ更为敏感、稳定、直观。本研究在猪口蹄疫和水泡病单抗联合诊断药盒的研制方面是一个有益的尝试。We used two ways of a monoclonal antibodybased DotELISA(McAbDotELISA)and a reverse indirect hemagglutination(RIHA)test to differentiate FMDV from SVDV .The results demonstrated that the McAbDotELISA technique had specific,rapid and simply advantages.In addition,the McAb-Dot-ELISA was more sensitive and stable than the RIHA.The test was a helpful try in developing a combined McAbs kit for diagnosing FMDV and SVDV.

关 键 词：猪口蹄疫病毒 水泡病病毒 免疫鉴别 诊断 

分 类 号：S858.285.3[农业科学—临床兽医学] S852.65[农业科学—兽医学]