玉米大斑病菌HT-毒素对玉米细胞VC氧化酶活性的影响  

Efffect of HT-toxin from Exserohilum turcicum onActivity of Ascorbate Oxidase in Corn Leaves

作　　者：董金皋[1,2] 闫淑娟[1,2] 韩建民[1,2] 崔海华[1,2] 赵光耀[1,2] 

机构地区：[1]河北农业大学植病系 [2]河北省正定县农业局

出　　处：《河北农业大学学报》1999年第1期50-53,共4页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金

摘　　要：玉米大斑病菌在体外培养可以产生对寄主玉米具有致毒活性的代谢产物———ＨＴ－毒素，它对玉米叶片细胞ＶＣ氧化酶的活性具有一定的抑制作用。表现为：ＨＴ－毒素处理后，供试的ＯＨ４３和ＯＨ４３Ｈｔ１２个玉米自交系的ＶＣ氧化酶活性虽然与ＣＫ处理的变化趋势一样，即在０～１２ｈ上升和在１２～４８ｈ下降，但比ＣＫ上升慢，下降快；而且，这种抑制作用还表现了一定的寄主选择活性。ＨＴ－毒素中加入强氧化剂Ｈ２Ｏ２后，对ＶＣ氧化酶的活性没有明显的提高和降低作用。HT-toxin,a toxic metabolite substance to corn,could be produced by Exserohilum turcicum in vitro,and it might inhibit the activity of ascorbate oxidase in corn leavesThe experimental results showed that the tendency of ascorbate oxidase activity in inbreds OH43 and OH43Ht1 was as the same as the control,but ascended more slowly during 012 h and descended more fast during 1248 h than control while exposing to HT-toxinThere was host-selectivity in this inhibiting effect among different combinations of toxin preparations and Ht gene of corn,and no obvious effect on ascorbate oxidase activity after H2O2 was added to HT-toxin

关 键 词：玉米 大斑病 病毒 VC氧化酶 HT-毒素 玉米细胞 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]