磷酸化信号转导与转录活化因子3和血管内皮生长因子及微血管密度在鼻息肉中的表达及意义  

Expression and significance of STAT3 and VEGF with MVD in the nasal polyps

作　　者：曹庆松[1] 张涛[1] 蒋立新[1] 王丽华[1] 罗思瑾[1] 

机构地区：[1]暨南大学附属第一医院耳鼻咽喉科,广州510630

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第14期641-644,647,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:检测磷酸化信号转导与转录活化因子3(p-STAT3)及血管内皮生长因子(VEGF)在鼻息肉组织中的表达和微血管密度(MVD)值,并探讨3者之间在鼻息肉发病过程中的相互关系。方法:选取符合纳入标准的鼻息肉患者手术切除标本30例(鼻息肉组),选取同期单纯行鼻中隔偏曲矫正术中切除的下鼻甲组织10例(对照组)。采用免疫组织化学SP法检测鼻息肉组和对照组p-STAT3、VEGF及MVD值。结果:p-STAT3在鼻息肉中黏膜上皮及腺体表达的阳性率为60%,鼻息肉组p-STAT3表达明显高于对照组(P<0.01)。VEGF在鼻息肉中黏膜上皮及腺体表达的阳性率为70%,鼻息肉组VEGF表达明显高于对照组(P<0.01)。鼻息肉组、对照组中MVD值分别为22.78±7.89、7.12±2.84。p-STAT3在鼻息肉中的表达与VEGF、MVD密切相关,随着p-STAT3强度的增加,VEGF分级及MVD值亦增加。结论:鼻息肉组织中p-STAT3、VEGF与MVD的表达明显增加,且3者之间呈正相关;p-STAT3与鼻息肉的血管生成密切相关,阻断STAT3通路即可阻断血管生成,阻止鼻息肉的形成、发生、发展及复发。Objective：To investigate microvessel density（MVD）and expressions of phosphor-STAT3 and vascular endothelial growth factor（VEGF）in nasal polyps,to explore their pathogenetic correlations.Method：All selected cases were measured up the standard of enrollment.Thirty samples of nasal polyps were obtained from patients undergoing nasal polypectomy,and 10 samples of inferior turbinate tissues were from patients undergoing nasal septal construction.p-STAT3、VEGF and MVD in nasal polyp tissues from 30 nasal polyposis patients and 10 samples of inferior turbinate tissues were detected with immunohistochemistry（sp）method.Result：In nasal polyp tissues,The positive rates of p-STAT3 and VEGF expression were 60% and 70% respectively,all were significantly higher in nasal polyps than in control inferior turbinates（P〈0.01）.MVD was significantly higher in nasal polyps than in control inferior turbinates（22.78±7.89 vs 7.12±2.84,P〈0.01）.p-STAT3 expression was positively correlated with VEGF expression and MVD.Conclusion：p-STAT3,VEGF and MVD were overexpressed in the tissues of nasal polyps,and p-STAT3 expression was positively correlated with VEGF expression and MVD;p-STAT3 was positively correlated with the neovascularization of nasal polyps,The inhibition of STAT3 pathway may inhibit the neovascularization,and then inhibit the formation and recurrence of the nasal polyps.

关 键 词：鼻息肉 磷酸化信号转导及转录活化因子3 血管内皮生长因子 微血管密度 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]