变应性鼻炎大鼠鼻黏膜中转化生长因子β1及成纤维细胞生长因子2表达的动态变化  

The dynamic expression of the TGF-β1 and FGF-2 in the nasal mucosa in the allergic rhinitis rat models

作　　者：谢冰斌[1] 刘月辉[2] 罗璝[2] 刘建国[2] 钟艳萍[1] 梅晓峰[1] 

机构地区：[1]佛山市中医院耳鼻咽喉科,广东佛山528000 [2]南昌大学第二附属医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第14期655-658,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：江西省自然科学基金项目(No:2007GZY1184);江西省教育厅课题(No:GJJ08112);江西省科技厅技撑计划(No:2009BSA10200)联合资助项目

摘　　要：目的:探讨变应性鼻炎发病过程中不同阶段,变应性鼻炎实验动物模型鼻黏膜转化生长因子β1(TGF-β1)、成纤维细胞生长因子2(FGF-2)表达的动态变化。方法:选健康SD大鼠180只,随机分成正常对照组(A组)和实验组(B、C组),每组60只。在实验的不同阶段取鼻黏膜进行免疫组织化学染色,检测TGF-β1、FGF-2的表达情况。结果:A组TGF-β1、FGF-2表达均呈阴性。B、C组均呈不同程度的阳性表达,且B组在避免变应原后表达有所下降,但未降至正常组水平(P<0.05)。结论:变应性鼻炎动物模型鼻黏膜TGF-β1、FGF-2的表达呈不同程度阳性,脱离变应原后表达下降。Objective：To investigate the dynamic expression of the TGF-β1 and FGF-2 in the nasal mucosa of rat models of allergic rhinitis,at different stages of the process during pathogenesis.Method：One hundred and eighty Sprague-Dawley（SD）rats were randomly divided into three groups：the normal Group A used for contrast and experimental groups：Group B and C,each group were 60 rats.Then,observed the expression of the TGF-β1 and FGF-2 in the nasal mucosa at different stages of the process by immunohistochemical staining.Result：The expression of the TGF-β1 and FGF-2 in the nasal mucosa of the normal Group A was negative,while the Group B and Group C were varying degrees of positive both,and Group B,the level of the TGF-β1 and FGF-2 were both decline after allergen avoidance,but still more notability than the expression o the Group B（P0.05）.Conclusion：The expression of the TGF-β1 and FGF-2 in the nasal mucosa of rats models were varying degrees of positive,and it is decline while the allergen avoidance.

关 键 词：鼻炎 变应性 动物模型 转化生长因子Β1 成纤维细胞生长因子2 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]