转耐辐射球菌irrE基因提高拟南芥盐胁迫耐受性的表型分析  

Expression of irrE in Deinococcus radiodurans can Enhance Salt Tolerance of Arabidopsis thaliana

作　　者：童仕波[1,2] 文国琴[1] 林敏[2] 杨毅[1] 李旭锋[1] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2010年第4期90-94,共5页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30971557);国家自然科学基金项目(30971816)资助

摘　　要：耐辐射球菌中的irrE基因作为一种全局性调节因子,在抵御极端辐射照射时起着至关重要的作用。利用农杆菌介导的花序浸染法,将irrE基因转化拟南芥,筛选和鉴定出表达irrE的植株,并分析了转基因植株的盐胁迫耐受能力。结果显示,转irrE拟南芥表现出对盐胁迫的耐受性提高;在盐胁迫下,转基因拟南芥体内脯氨酸含量比野生型有明显的提高。RNA半定量分析显示转基因植株中AtNHX1表达水平升高。因此推测irrE基因在转基因拟南芥中调控了AtNHX1的表达,引起了植株的盐胁迫耐受性提高。As a global regulator,the irrE gene is responsible for extreme radiation resistance.irrE gene was introduced into Arabidopsis plants via agrobacterium-mediated transformation.The transgenic plants were selected and their salt tolerance was analyzed.The results showed that irrE gene could enhance salt tolerance in Arabidopsis thaliana,as well as increase the free proline content in transgenic plants under salt stress.The semi-quantitative RT-PCR analysis at transcriptional level showed AtNHX1 expression quantities rose in transgenic plants under salt stress.These results demonstrated the function of irrE in Arabidopsis thaliana is increasing the expression level of AtNHX1,thus enhancing salt tolerance of Arabidopsis thaliana.

关 键 词：转基因拟南芥 irrE基因 盐胁迫 

分 类 号：Q786[生物学—分子生物学]