玉米胚性愈伤组织的遗传转化及耐草甘膦植株再生  

Genetic Transformation of Maize Embryonic Callus and Regeneration of Glyphosate-tolerant Plant

作　　者：余桂容[1,2] 杜文平[2] 宋军[2] 窦茜[2] 刘永胜[1] 陆伟[3] 徐利远[2] 

机构地区：[1]四川大学生命科学院,成都610064 [2]四川省农业科学院生物技术核技术研究所,成都610066 [3]中国农业科学院生物技术研究所,北京100081

出　　处：《中国农业科技导报》2010年第4期108-113,共6页Journal of Agricultural Science and Technology

基　　金：转基因生物新品种培育重大专项(2009ZX08003-003B);四川省农科院优秀论文基金项目(2008-2010转耐草甘膦抗虫玉米研究)资助

摘　　要：以优良玉米自交系X2211的幼胚胚性愈伤组织为受体材料,采用农杆菌介导法将来自微生物荧光假单胞杆菌(Pseudomonas fluorescens)G2菌株的抗除草剂基因2mG2-epsps转入玉米愈伤组织细胞,转化后的细胞经草甘膦筛选,获得抗性愈伤组织并再生植株282株,成活86株,经特异PCR检测表明其中26株植株转入了2mG2-epsps基因,喷施0.125%除草剂试验检测表明,转基因植株具有良好的耐除草剂特性,为抗除草剂玉米新品种选育提供了较好的亲本材料。2mG2-epsps gene from Pseudomonas fluorescens G2 was transferred to immature embryo-derived callus of the elite maize inbred line X2211 via Agrobacterium-mediated transformation.282 transformed plants were regenerated from glyphosate-resistant callus,86 plants of which were grown well in the field.Glyphosate resistance and allele-specific PCR analysis demonstrated that the 2mG2-epsps gene was transferred into the maize genome in 26 regeneration plants.The plants showed resistance after spraying 0.125% glyphosate,which indicated the expression of 2mG2-epsps gene in the plants.Some of the transgenic plants gained high tolerance to glyphosate.This study has provided good parental materials for breeding new glyphosate-resistant maize varieties.

关 键 词：玉米幼胚 遗传转化 草甘膦 2mG2-epsps基因 

分 类 号：Q758[生物学—分子生物学]