不同N、P水平下接种摩西球囊霉对白三叶草中C∶N∶P比和RNA含量的影响  

The influences of arbuscular mycorrhizal fungus,Glomus mosseae,on C:N:P ratios and RNA contents of white clover at different nitrogen and phosphorus addition levels

作　　者：陈梅梅[1,2] 阴红彬[2] 王幼珊 

机构地区：[1]中国石油安全环保技术研究院,北京100083 [2]中国科学院生态环境研究中心,北京100085 [3]北京市农林科学院植物营养与资源研究所,北京100089

出　　处：《生态学报》2010年第15期4093-4102,共10页Acta Ecologica Sinica

基　　金：中国石油天然气股份有限公司科学研究与技术开发资助项目(2008D-4704-1);国家重点基础研究发展计划(2007CB407301);中国科学院知识创新工程重大项目(KZCX1-YW-06-03)

摘　　要：通过室内盆栽试验研究了不同N、P水平下接种摩西球囊霉(Glomus mosseae)对三叶草中C:N:P比和RNA含量的影响。试验结果表明,(1)在外加P浓度为50mgkg-1或低于50mgkg-1时,随着外加P浓度的增加,三叶草的菌根侵染率增加,而且接种摩西球囊霉增加了三叶草的生物量,提高了其P含量和C/N比,降低了C/P和N/P比;外加P浓度为100mgkg-1时,接种摩西球囊霉对三叶草生物量和C:N:P比无显著影响;对于非菌根三叶草,其RNA含量与P含量成正相关;(2)外加P浓度的增加提高了三叶草生物量、RNA含量和对P养分的吸收,抑制了三叶草对N的吸收;外加N浓度的增加降低了三叶草的生物量、P含量和RNA含量,增加了三叶草中的N含量;(3)三叶草中的C、N和P含量之间显著相关,表明植物中元素组成的变化是相互联系、相互影响的;(4)三叶草中的N/P比受土壤N/P供给比调节,即随着土壤N/P供给比的变化,三叶草中N/P比也相应变化。A pot experiment was conducted to reveal the effects of an arbuscular mycorrhizal fungus,Glomus mosseae,on carbon（C）：nitrogen（N）：phosphorus（P） ratios and RNA contents of clover plants at different N and P addition levels.The experimental results indicated that Glomus mosseae colonization increased the biomass,P concentrations and C/N ratios,while decreased N concentrations,C/P and N/P ratios,under P addition level of or lower than 50 mg kg^-1.Under P addition level of 100 mg kg^-1,Glomus mosseae colonization showed no significant effects on the biomass,nutrient concentrations and C：N：P ratios of clover plants.Generally Glomus mosseae colonization increased RNA contents of clover plants compared with non-mycorrhizal controls.For non-mycorrhizal clover,P concentrations were positively correlated with RNA contents,indicating the changes in P concentrations can alter RNA contents of clover plants.C concentrations were significantly related to N,P concentrations in clover plants,suggesting that the changes in C concentrations depended on N and P concentrations.Furthermore,N/P ratios of clover plants were also mediated by N/P supply ratios in soil.

关 键 词：N/P供给比 摩西球囊霉(Glomus mosseae) C∶N∶P计量比 RNA 

分 类 号：S541.2[农业科学—作物学]