催化动力学光度法测定痕量铁(Ⅲ)及机制探讨  

Catalytic Kinetic Spectrophotometric Determination of Trace Iron (Ⅲ) and Its Reaction Mechanism

作　　者：葛笑兰[1] 张振新[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,安徽淮北235000

出　　处：《中国食品卫生杂志》2010年第4期326-328,共3页Chinese Journal of Food Hygiene

基　　金：安徽省教育厅自然科学基金(2006KJ163C)

摘　　要：目的建立了测定痕量铁的双波长双指示剂催化动力学光度分析的新方法。研究在盐酸介质中,铁(Ⅲ)催化过氧化氢氧化亚甲基蓝使其褪色反应的最佳条件及探讨其反应机制。方法通过测量催化体系和非催化体系在610nm和666nm下吸光度的变化,用双波长指示催化动力学光度法以测定痕量铁(Ⅲ)。结果线性范围为0.2～3.0μg/25ml,检出限为9.24×10-11g/ml。结论本方法灵敏度高,体系稳定,操作简便,用于实际样品中铁的测定,结果较为满意。Objective To establish a dual-indicator catalytic kinetics spectrophotometric method for the detection of trace amounts of iron （Ⅲ）.The test was based on the remarkable catalytic effect of iron （Ⅲ） on the oxidation of methylene blue by hydrogen peroxide in hydrochloric acid medium.Method Determining the absorbance at 610 nm and 666 nm for catalytic and uncatalytic systems with the dual-wavelength catalytic spectrophotometric method to detect trace amount of iron （Ⅲ）.The best experimental condition and the reaction mechanism were studied.Results The linear range was 0.2-3.0 μg/25 ml.The detection limit was 9.24 ×10-11 g/ml.Conclusion This method was sensitive,stable and easy operated.The practical use on the detection of trace iron in tea samples was satisfactory.

关 键 词：铁 催化动力学光度法 亚甲基蓝 反应机制 

分 类 号：O657.32[理学—分析化学]