白细胞介素┐1α诱导脑血管内皮细胞与单核细胞的粘附和迁移及药物拮抗作用  

Inhibitory effects of drugs on monocyte adhesion and transmigration through interleukin 1 alpha induced cerebromicrovascular endothelial cell in vitro

作　　者：储智勇[1] 芮耀诚[1] 张蕾 马金刚 

机构地区：[1]上海第二军医大学药学院药理学教研室

出　　处：《中国药理学通报》1999年第1期35-38,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：目的研究细胞因子ＩＬ－１α影响单核细胞（ＭＮＣ）与体外培养的牛脑微血管内皮细胞（ＢＣＭＥＣ）粘附、迁移的动力学过程及药物的保护作用。方法利用培养在胶原层上的内皮细胞单层，对粘附于内皮细胞单层上的ＭＮＣ及胶原层中的ＭＮＣ计数，计算ＭＮＣ粘附率及迁移率。结果ＩＬ－１α（５００ｋＵ·Ｌ－１）可促进ＭＮＣ与ＢＣＭＥＣ的粘附与迁移，其促粘附与迁移作用具明显的时效关系。ＩＬ－１α促进粘附达最大效应时间为２ｈ，粘附率达３７．３％，较对照提高６２．６％。ＩＬ－１α促迁移作用２ｈ达到坪值，迁移率为１７．４％，较对照提高１４１．７％。药物欧芹素乙（ｉｍｐｅｒａｔｏｒｉｎ，ＩＭＰ）、已酮可可碱（ｐｅｎｔｏｘｉｆｙｌｉｎｅ，ＰＴＸ）对ＩＬ－１α引起的ＭＮＣ与ＢＣＭＥＣ的粘附与迁移有抑制作用，且呈剂量依赖性。在浓度为１、１０及１００μｍｏｌ·Ｌ－１时，其粘附抑制率分别为３７．７％、４２．０％、６６．０％和３８．３％、６４．２％、８７．０％；迁移抑制率分别为３２．８％、６６．４％、７０．７％和３８．８％、５５．２％、７４．１％。结论ＩＭＰ与ＰＴＸ呈剂量依赖性抑制ＭＮＣ与ＩＬ－１α诱导的ＢＣＭＥＣ的粘附及迁移。AIM To study drugs antagonism of monocyte adhesion to and transmigration through interleukin 1α(IL 1α) stimulated bovine cerebromicrovascular endothelial cell(BCMEC)layers in vitro . METHODS The adhesion and migration of bovine monocyte were measured using a model system comprising BCMEC cultured on colagen gels in vitro . RESULTS Prestimulation of BCMEC with IL 1α(500 kU·L -1 )enhanced monocytes adhesion to and transmigration through IL 1α stimulated BCMEC layers.Both adhesion and transmigration of monocyte were increased with time. After 2 h incubation of BCMEC with IL 1α,adhesion or migration reached maximum(37 3% and 17 4% respectively). At concentrations fron 1 to 100 μmol·L -1 ,both IMP and PTX were found to inhibit the monocyte adhesion and migration. Their maximal adhesion inhibitory effects were 66 0% and 87 0% at 100 μmol·L -1 ,respectively, and their maximal migration inhibitory effects 70 7% and 74 1% at 100 μmol·L -1 ,respecively. CONCLUSION Both IMP and PTX may decrease monocyte adhesion and transmigration through IL 1α induced BCMEC layers.

关 键 词：动脉粥样硬化 IL-1 已酮可可碱 MNC 欧芹素乙 

分 类 号：R543.5[医药卫生—心血管疾病] R972[医药卫生—内科学]