几种香茶菜属二萜化合物对自由基导致线粒体损伤的保护作用  

Protective effect of diterpenoid compounds on liver mitochondria injury induced by free radicals

作　　者：张予[1,2] 王筠[1,2] 徐霞[1,2] 戈士文[1,2] 张覃沐[1,2] 

机构地区：[1]河南省医学科学研究所药理室 [2]河南医科大学实验中心

出　　处：《中国药理学通报》1999年第1期46-48,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金;河南省自然科学基金

摘　　要：目的了解香茶菜属二萜类化合物冬凌草甲素（Ｏｒｉｄ）、腺花香茶素（Ａｄｅ）、黄花香茶菜素（Ｓｃｕｌ）是否具有抗氧化作用。方法用分光光度法测定脂质过氧化物丙二醛（ＭＤＡ）含量及线粒体肿胀度。用荧光分光光度法测定线粒体膜流动性。结果Ｏｒｉｄ，Ａｄｅ，Ｓｃｕｌ４０，８０，１６０μｍｏｌ·Ｌ－１抑制铁－半胱氨酸（Ｆｅ２＋－Ｃｙｓ）引起的肝线粒体ＭＤＡ形成，并呈剂量依赖关系。Ｏｒｉｄ，Ａｄｅ，Ｓｃｕｌ１６０μｍｏｌ·Ｌ－１抑制肝线粒体膜流动性下降（Ｐ值分别为２．２９７±０．０２２，０．３８９±０．００９，０．３８２±０．０１３，Ｆｅ２＋－Ｃｙｓ的Ｐ值为０．４２３±０．０１４）；Ａｄｅ１６０μｍｏｌ·Ｌ－１还可抑制脂质过氧化引起的肝线粒体肿胀。结论Ｏｒｉｄ，Ａｄｅ。AIM To study the protective effect of diterpenoid compounds on the mitochondria injury induced by free radical. METHODS TBA method was used to measure the content of MDA spectrometric method to measure the turbidity of mitochondria suspension. fluorescence anisotropy assay were used to measure the mitochondrial membrane fluidity. RESULTS Oridonin (Orid), adenanthin (Ade) and sculponeatin (Scul) 40, 80 and 160 μmol·L -1 inhibited MDA formation of liver mitochondria in a concentration dependent manner. Orid, Ade and Scul 160 μmol·L -1 inhibited the increase of P value (0 297±0 022, 0 389±0 009 and 0 382±0 013 vs 0 423±0 014 in ferrous cysteine group) Ade 160 μmol·L -1 prevented the decrease of the absorbane induced by ferrous ascorbic acid in liver mitochondria. CONCLUSTON Orid, Ade and Scul all have antioxidative effect through diminishing mitochondria lipid peroxidation induced by free radicals.

关 键 词：香茶菜属 二萜类化合物 线粒体损伤 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学] R977.9[医药卫生—中医学]