丹皮总苷体外对三类免疫细胞功能的影响  

Effects of total glucosides of mudan cortex on functions of three types of immune cells in vitro

作　　者：王斌[1] 葛志东[1] 周爱武[1] 陈敏珠[1] 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理研究所

出　　处：《中国药理学通报》1999年第1期63-65,共3页Chinese Pharmacological Bulletin

基　　金：安徽省科委"八五"攻关研究项目

摘　　要：目的研究丹皮总苷（ＴＧＭ）体外对３类免疫细胞功能的影响。方法采用［３Ｈ］－ＴｄＲ参入法，检测Ｔ淋巴细胞增殖反应和分泌白介素２（ＩＬ－２）活性，Ｂ淋巴细胞增殖反应以及巨噬细胞产生ＩＬ－１。结果ＴＧＭ２～５０ｍｇ·Ｌ－１可明显促进刀豆蛋白Ａ诱导小鼠Ｔ淋巴细胞增殖反应和大鼠Ｔ淋巴细胞产生ＩＬ－２，ＴＧＭ还可促进脂多糖诱导Ｂ淋巴细胞增殖反应以及大鼠腹腔巨噬细胞产生ＩＬ－１，它们的浓度－效应曲线呈钟罩形。结论ＴＧＭ具有浓度依赖性双向免疫调节作用。AIM To study the effects of total glucosides of mudan cortex (TGM) on functions of three types of immune cells in vitro . METHODS T lymphocyte proliferation and its interleukin 2 (IL 2) secretion, B lymphocyte proliferation, IL 1 production by peritoneal macrophages were measured by TdR incorporation. RESULTS TGM 2￣50 mg·L -1 could significantly promote murine T lympocyte proliferation and IL 2 secretion by rat T lymphocyte induced by concanavalin A, and murine B lymphocyte proliferation as well as IL 1 production of rat peritoneal macrophages induced by lipopolysaccharide. Their concentration effect curves showed the bell shaped. CONCLUSION TGM has a dual immunomodulatory activity in a concentration related way.

关 键 词：丹皮总苷 T细胞 B细胞 巨噬细胞 免疫功能 

分 类 号：R285.5[医药卫生—中药学]