P物质体外对骨髓CFU-GM和CFU-C21的影响  

The effect of in vitro substance P on bone marrow CFU-GM and CFU-C21

作　　者：夏长青[1] 王逸兰[1] 陆道培[1] 

机构地区：[1]北京医科大学人民医院血液病研究所

出　　处：《北京医科大学学报》1999年第2期141-142,共2页Journal of Peking University(Health Sciences)

摘　　要：目的：探讨Ｐ物质与造血的关系。方法：采用甲基纤维素半固体造血祖细胞培养法检测正常血浓度的ＳＰ以及不同浓度的ＳＰ对骨髓ＣＦＵ－ＧＭ和ＣＦＵ－Ｃ２１的作用。结果：１０＾－１０ｍｏｌ．Ｌ＾－１和１０＾－１１ｍｏｌ．Ｌ＾－ＳＰ组ＣＦＵ－ＧＭ的产率分别为１９３±１９和２０６±４０，与对照相比具有显著差异；１０＿１６－ｍｏｌ．Ｌ＾－１和１０＾－１１ｍｏｌ．Ｌ＾－１ＳＰ对ＣＦＵ－Ｃ２１也有明显刺激作用。To investigate the relationship between substance P(SP) and hematopoiesis. Methods: The role of SP with normal blood concentration(10^(-11)mol· L^(-1) and various concentrations higher or lower than 10^(-11)mol·L^(-1)were assessed in the semi-solid methyl-cellulose hematopoietic cell culture system. Results: SP with 10^(-10) mol· L^(-1)increased significantly CFU-GM (193±19 ) and CFU-C_(21) (143±16) compared with control(CFU-GM: 137±17 and CFU-C_(21): 80 ±31 respectively, P <0. 05). 10^(-11)mol· L^(-1) SP had similar effect on CFU-GM(206 ±40 ) and CFU-C_(21_ (153±15 ) to that of 10^(-10) mol· L^(-1) SP compared with control (P < 0. 05 ). The effect of SP with more than 10(^-10) mol· L^(-1) or less than 10^(-11) mol· L^(-1) had low effect on CFU-GM and CFU-C(_21) comparable to the control(P > 0. 05 ). Conclusion: SP with proper concentration can stimulate bone marrow CFU--GM and CFU-C_(21) in vitro and probably SP plays important part in the regulation of normal bone marrow hematopoiesis.

关 键 词：P物质 骨髓 CFU-GM CFU-C21 

分 类 号：R331.22[医药卫生—人体生理学] R551.3[医药卫生—基础医学]