两种方法建立大鼠子宫内膜异位症模型的探讨  

Two different ways of rat endometriosis models by autotransplantation and opening uterine cavity

作　　者：张晟宁[1] 肖成明[1] 哈春芳[2] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2010年第8期679-681,F0002,共4页Ningxia Medical Journal

摘　　要：目的探讨在同一大鼠体内采用2种不同方法建立自体大鼠子宫内膜异位症模型的成模率及异位内膜的临床特征。方法随机抽取16只雌性未交配性成熟大鼠,采用内膜移植法将同一大鼠右侧子宫内膜组织移植于腹壁;采用开放宫腔法将左侧宫腔内膜直接开放于宫骶韧带处。术后第28d开腹比较两组建模情况;取出异位组织进行组织形态学和病理学观察。结果移植法建模成功率为87.5%,开放法建模成功率为62.5%;肉眼观移植法成模后的异位内膜呈隆起紫兰色或干酪样物为主,病灶与周围组织几乎无黏连;宫腔开放法异位灶体积明显增大,局部呈隆起暗红色或透亮的小囊状,异位灶与周围组织黏连严重;术后病理检查与肉眼观察符合率移植法为87.5%,开放法为71.43%,两种方法联合建模符合率为100%。结论自体子宫内膜移植法及开放宫腔法均可成功建立内异症大鼠模型而且移植法优于开方法;两种方法结合可提高建模成功率。Objective To Analyze two different ways of rat endometriosis models by autotransplantation and opening uterine cavity,and observe clinical features of ectopic endometrium. Methods Sixteen female SD rats were autotransplanted of uterine tissue intoright side of the abdominal wall,at same time,the uterine tissue from left side of the uterine cavity were exposed to utserosacral ligament through surgery in same rat. In order to determine whether the models were established successfully,hetero-topic tissues were taken for histomorphological and pathological examination on the 28th day after surgery. Results The success ratio of rat endometriosis model by way of autotransplantation was 87. 5% as well as the 62. 5% in the model by opening uterine cavity. The ectopic endometrium by autotransplantation was higher than that by opening uterine cavity. Its color was hyacinthine or caseating almost without adhereing to peripheral tissue. The ectopic endo-metrium by opening uterine cavity was bigger,locally with dark red,and rounded hump or lucent sacculiform. And the lesion was adherent seriously. The rate of accord examined by pathological examination and the unaided eye： endometriosis of autotransplantation was 87. 5% ; opening uterine cavity was 71. 43% ; and the combination of the two ways was 100% . Conclusions Endometriosis models by the way of the autotransplantation and opening uterine cavity are both the effective ways for establishing rat endometriosis models. Combined methods can increase the success rate of constructing rat models.

关 键 词：自体子宫内膜异位症模型 大鼠 临床特征 

分 类 号：R711.71[医药卫生—妇产科学]