心肌营养素-1对血管紧张素Ⅱ诱导心肌细胞肥大的影响  

Effect of cardiotrophin-1 on hypertrophy of cardiocytes induced by angiotensin Ⅱ

作　　者：杨爽[1] 杨凯[2] 陆莹[1] 于波[1] 

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086 [2]哈尔滨理工大学医院

出　　处：《心血管康复医学杂志》2010年第4期346-349,358,F0004,共6页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：黑龙江省青年基金QC06C053;哈尔滨医科大学附属第二医院青年启动基金QN2007-07;黑龙江省卫生厅课题2006-141;省部共建黑龙江省教育部心肌缺血机理与诊疗技术重点实验室

摘　　要：目的：研究心肌营养素-1（CT-1）对血管紧张素Ⅱ（AngⅡ）诱导的心肌细胞肥大的影响.方法：利用AngⅡ刺激心肌细胞,导致细胞肥大,应用CT-1反义脱氧寡核苷酸进行干预.检测心肌细胞大小及3H-亮氨酸掺入率的变化;以逆转录聚合酶链反应（RT-PCR）检测CT-1 mRNA及β-肌球蛋白重链（β-MHC） mRNA表达,免疫组织化学法检测心肌细胞CT-1蛋白的表达. 结果：经AngⅡ刺激后,在相差显微镜下可见心肌细胞面积变大,3H-亮氨酸掺入率、CT-1蛋白的表达增高;CT-1和β-MHC mRNA水平的表达增加.利用Fugene 6 可将CT-1反义脱氧寡核苷酸转染入心肌细胞.CT-1反义脱氧寡核苷酸组心肌细胞面积较肥大组明显减小[（81.257±3.995）mm2∶（ 127.214±5.693） mm2],3H-亮氨酸掺入量[（1653.33±17.91）cpm∶（1971.50±42.16） cpm]及CT-1蛋白[（3.16±0.17） %∶（3.51±0.29）%]明显减少;CT-1[（0.2137±0.0227）∶（0.4023±0.0160）]和β-MHC mRNA[（0.5032±0.0261）∶（0.773 4±0.0486）]表达亦显著减少（P〈0.05-〈0.01）.各指标Fugene 6组、错义组与肥大组无明显差异.结论：AngⅡ致心肌细胞肥大过程中伴有CT-1表达增加,应用CT-1反义脱氧寡核苷酸后可使之下降,说明CT-1参与了心肌细胞的肥大机制.Objective： To study the effect of cardiotrophin- 1 （CT-1） on hypertrophy of cardiocytes induced by angiotensin Ⅱ （Ang Ⅱ ）. Methods： The cultured cardiocytes were treated with AngⅡ to induce hypertrophy and given with CT-1 antisense oligodeoxyribonucleotide （ASODN）. The cell size and 3H-leucine incorporation were examined. The mRNA levels of CT-1 and β-myosin heavy chain （β-MHC） were examined by real-time PCR method. The protein expressions of CT- 1 were examined by immunohistochemistry method. Results ： Stimulated by Ang It , the cardiocytes were enlarged under the contrast phase microscope. After stimulated by Ang Ⅱ , 3H- leucine incorporation and the protein expression of CT- 1 increased in the hypertrophic cardiocytes. The mRNA levels of CT- 1 and 3- MHC increased also. Compared with hypertrophy group, the area of cardiocytes [- （ 127. 214±5. 693） mm^2 vs. （81. 257±3. 995） mm^2], 3H-leueine incorporation [ （1971.50±42.16） cpm vs. （1653.33± 17.91） epm], protein expression of CT-1 [（3.51±0. 29）% vs. （3.16±0.17） %], CT-1 mRNA [ （0. 4023±0. 0160） vs. （0. 2137±0. 0227）] and β-MHC mRNA [- （0. 773 4±0. 0486） vs. （0. 5032±0. 0261）1 significantly decreased in CT-1 ASODN group （given by CT- 1 ASODN）, P〈0.05 - 〈0.01. These indexes were no significantly difference among CT- 1 MSODN （missence oligodeoxyrihonucleotide） group, hypertrophy group and Fugene 6 group. Conelusiom Ang Ⅱmay induced the cardiocytes hypertrophy. The expression of CT-1 increased in hypertrophic cardiocytes stimulated by Ang Ⅱ. The CT- 1 ASODN inhibited the increased expression of CT- 1 in hypertrophic cardiocytes stimulated by Ang Ⅱ.

关 键 词：血管紧张素Ⅱ 心肌 寡脱氧核糖核苷酸类 反义 

分 类 号：R541.75[医药卫生—心血管疾病]