气虚血瘀证(心肌缺血)大鼠模型单核细胞炎性因子表达的相关性探讨  

Study on Mononuclear Cells Associated with The Expression of InflammatoryFactors in Rats with Qi Deficiency and Blood Stasis Syndrome( Myocardial Ischemia)

作　　者：陈婵[1] 郭淑贞[1] 王伟[1] 仇琪[1] 王娟[1] 李春[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中医药学报》2010年第4期16-19,共4页Acta Chinese Medicine and Pharmacology

基　　金："重大新药创制"科技重大专项(编号:2009ZX09502-018);国家重点基础研究发展计划(973计划)项目(No.2003CB517105)

摘　　要：目的:探讨气虚血瘀证与单核细胞(PBMC)的相关性及其可能的炎症机制。方法:复制心梗气虚血瘀证动物模型,采用半定量RT-PCR技术检测模型组及假手术组动物术后第7天、14天、28天外周血单核细胞TNFα、IL-1β、IL-6、MCP-1的表达情况。结果:不同时间点模型组动物单核细胞TNFα、IL-1β、IL-6、MCP-1的表达均高于假手术组动物(P<0.05)。结论:气虚血瘀证(心肌缺血)与单核细胞密切相关,可能成为该证候重要的微观指标。Objective： To explore the relationship between Qi deficiency and blood stasis syndrome and mononuclear cells of peripheral blood（PBMC）,and investigate the possible inflammatory mechanisms.Methods： The rat model of Qi deficiency and blood stasis syndrome based on myocardial infarction was established,the expression of TNFα、IL-1β、IL-6、MCP-1 in the PBMC was measured by semi-quantitative RT-PCR between model group and sham-operated group at 7 days、14 days、28 days respectively after operation.Result： The expression of TNFα、IL-1β、IL-6、MCP-1 in the PBMC of model group was significantly higher than that control group（P 0.05） at different times.Conclusion：Qi deficiency and blood stasis syndrome（myocardial ischemia） closely associated with PBMC,the PBMC may becomes important microscopic indice of this syndrome.

关 键 词：气虚血瘀证 动物模型 单核细胞 炎性因子 

分 类 号：R241.3[医药卫生—中医诊断学]