黄连提取物对角质形成细胞增殖活性的影响  

Effect of Coptis Chinensis Extract on Proliferation of Keratinocytes

作　　者：赵茉[1] 高莉 彭晓明 祝建波[1] 闫明 

机构地区：[1]石河子大学生命科学学院,新疆石河子832000 [2]新疆维吾尔医药研究所,新疆乌鲁木齐830049

出　　处：《中国中医药信息杂志》2010年第8期22-24,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技支撑计划(2007BAI48B00);新疆维吾尔自治区卫生厅青年科技人才专项科研项目(2009Y11)

摘　　要：目的探讨黄连提取物对角质形成细胞株HaCaT增殖的影响及其机制。方法将不同浓度的黄连提取物作用于培养的HaCaT细胞,采用台盼蓝染色法检测细胞活力;光镜观察细胞的形态变化;MTT比色法测定药物对细胞增殖的影响;RT-PCR半定量检测角蛋白K6和原癌基因Bcl-2的mRNA表达水平变化。结果不同浓度(10.46～167.3μg/mL)黄连提取物处理HaCaT细胞24～72h后,细胞增殖受到明显抑制,抑制率最高达75.5%,且呈现一定的浓度依赖性和时间依赖性。黄连提取物可以下调HaCaT细胞内的角蛋白K6和Bcl-2 mRNA转录水平,随着浓度的增高,诱导其表达水平降低的趋势越明显,呈现一定的浓度依赖性。结论黄连提取物可以明显抑制HaCaT细胞的增殖,其机制可能与下调角蛋白K6和原癌基因Bcl-2的表达有关。Objective To study the effects of Coptis Chinensis extract on the proliferation of HaCaT cellsand related mechanisms. Methods Cultured HaCaT cells were treated with different concentrations ofCoptis Chinensis extract, and cell viability was detected by Trypan staining. Cell morphology changes wereobserved under optical microscopy. HaCaT cells poliferation were deteced by MTT assay. The mRNAexpression changes of keratin 6 and Bcl-2 were separately detected by semi-quantitative reversetranscription PCR. Results After incubation with different concentrations （10.46～167.3 μg/mL） of CoptisChinensis extract for 24 to 72 h, HaCaT cells were inhibited dramatically and the highest inhibition ratewas 75.5%. The mRNA expession of keratin 6 and Bcl-2 was down-regulated and its effect was exerted in adose-dependent manner. Conclusions Extract of Coptis Chinensis can inhibit the proliferation of HaCaT cell.It might be related to the down-regulation of keratin 6 and Bcl-2 expression.

关 键 词：黄连 HACAT 细胞 角蛋白K6 BCL-2 

分 类 号：R285.5[医药卫生—中药学]