RP-HPLC法测定复方金蒲片中延胡索乙素的含量  

RP-HPLC Determination of Tetrahydropalmatine in Compound Jinpu Tablets

作　　者：黄明立[1] 黄东萍[1] 蒋贵发[1] 刘宗河 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]广西肿瘤研究所,广西南宁530021

出　　处：《中国中医药信息杂志》2010年第8期45-46,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立复方金蒲片中延胡索乙素的含量测定方法。方法采用高效液相色谱法,色谱柱为Hypersil BDS-C18,流动相为甲醇-5%乙酸(40:60),并用浓氨试液调至pH4.7,流速1.0mL/min,柱温为25℃,检测波长280nm。结果延胡索乙素浓度在0.436～2.180μg范围内峰面积与浓度呈良好线性关系,r=0.9997,平均回收率为98.4%,延胡索乙素与其相邻杂质峰的分离度符合要求。结论本方法简便、专属、重复性好,可用于延胡索乙素的定量分析。Objective To develop a method for determination of tetrahydropalmatine in Compound JinpuTablets. Methods RP-HPLC method was used with Hypersil BDS-C18 column, the mobile phase wasmethanol-5% acetic acid （40∶60） adjusted pH to 4.7 by ammonia, flow rate was 1.0 mL/min, and columntemperature was 25 ℃, with the detection wavelength at 280 nm. Results The linear correlation wasobserved for the concentration of 0.436～2.180 μg （r =0.999 7）. The average recovery was 98.4%. Resolutionbetween tetrahydropalmatine and other peaks met the requirements. Conclusion The method is convenient,selective and reproducible for quantitative analysis of tetrahydropalmatine.

关 键 词：复方金蒲片 延胡索乙素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]