靶向Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1反义核酸抑制消化系肿瘤细胞增殖效果的差异  

The difference of proliferatory inhibition effects of Bcl-2,Bcl-xl, Mcl-1,Bcl-w and A1 antisense oligodeoxynucleotides on gastrointestinal cancer cells

作　　者：蒋建伟[1] 吴风云[1,2] 何金花[1,3] 廖晓莉[1] 王威[1] 吴志慧[1] 

机构地区：[1]暨南大学医学院生化教研室,广东广州510630 [2]浙江省温州市平阳县人民医院,浙江温州325400 [3]广东省广州市番禺区中心医院检验科,广东广州511440

出　　处：《中国药理学通报》2010年第8期1093-1098,共6页Chinese Pharmacological Bulletin

基　　金：广东省科技计划资助项目(No2009B080701051);广东省医学科研资助基金(NoA2009348)

摘　　要：目的比较靶向Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1反义核酸(antisense oligodeoxynucleotide,ASO)对消化系统肿瘤细胞(肝癌HepG2细胞、胃癌MGC-803细胞、结肠癌Lovo细胞)的增殖抑制作用和致凋亡作用的差异。方法分别合成靶向Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1的反义核酸和随机序列反义核酸(randomolig odeoxynucleotide,RODN),采用脂质体Li-pofectamineTM 2000转染细胞,WST法检测相同浓度的5种ASOs对肝癌HepG2细胞、胃癌MGC-803细胞、结肠癌Lovo细胞的增殖抑制作用和致凋亡作用。结果 Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1等5种ASOs中,不论是对细胞增殖抑制还是致凋亡作用,均以Bcl-xlASO、Mcl-1ASO的作用效果较好。结论在Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1等5种ASOs中,Bcl-xlASO、Mcl-1ASO可明显抑制消化系肿瘤细胞增殖,诱导细胞凋亡。Aim To exam the difference in proliferatory inhibition effects of Bcl-2,Bcl-xl,Mcl-1,Bcl-w and A1of Bcl-2 proteins family members antisense oligodeoxynucleotides on gastrointestinal cancer cells （ HepG2, Lovo,MGC-803） . Methods The antisense oligodeoxynucleotide targeting for Bcl-2,Bcl-xl,Mcl-1,Bcl-w and A1 and a random oligodeoxynucleotide （ RODN） were synthesized and transfected into gastrointestinal cancer cells （ HepG2,Lovo,MGC-803 ） using LipofectamineTM2000 for 48 hours,cell proliferation inhibition was determined by WST method and cell apoptotic level examined by flow cytometry. Results Antisense oligodeoxynucleotide targeting Bcl-xl and Mcl-1 gene could suppress the growth of gastrointestinal cancer of HepG2,Lovo,MGC-803 cells and induce apoptosis of these cells significantly （ P〈0. 05） . The other antisense oligodeoxynucleotide targeting Bcl-2,Bcl-w and A1 showed an indistinctively effect on inhibiting cell proliferation and inducing cell apoptosis. Conclusion Antisense oligodeoxynucleotide targeting Bcl-xl and Mcl-1 can inhibit proliferation and induce apoptosis of gastrointestinal cancer cells of HepG2,Lovo,MGC-803 cells in vitro.

关 键 词：反义核酸 BCL-2 BCL-XL MCL-1 增殖 凋亡 肿瘤细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]