菝葜提取物对Hut-78细胞Bcl-2基因转录水平的影响  

The Effect of Extraction of Smilax china L. on Transcription Level of Bcl-2 Gene in Hut-78 Cells

作　　者：高莉 彭晓明 霍仕霞 赵茉[2] 唐晓琴 闫明 

机构地区：[1]新疆维吾尔医药研究所,乌鲁木齐830049 [2]石河子大学生命科学学院,832003

出　　处：《医药导报》2010年第8期993-995,共3页Herald of Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(基金编号:2009211B38);国家科技支撑计划项目(基金编号:2007BAI48B06)

摘　　要：目的探讨菝葜提取物对Hut-78细胞Bcl-2基因转录水平的影响。方法利用半定量逆转录-聚合酶链反应(RT-PCR)测定给药后各组的吸光度值,计算各组的Bcl-2/GAPDH值,从而分析Bcl-2基因转录水平的变化。结果菝葜提取物对Bcl-2基因的转录水平具有下调作用,药物浓度在8μg·mL-1时的效果最为明显。结论该菝葜提取物可降低Hut-78细胞的Bcl-2基因的转录。Objective To investigate the effect of Smilax china L. extraction on the transcription level of Bcl-2 gene in Hut-78 cells. Methods The light density of of each group by semi-quantitative RT-PCR was measured after being treated,to analyze the change of the transcription level of Bcl-2 gene by calculating Bcl-2 /GAPDH. Results The extraction of Smilax china L inhibited the transcription level of Bcl-2 gene,and showed the best at a concentration of 8 μg·mL-1. Conclusion The extraction of Smilax china L could reduce the transcription level of Bcl-2 gene in Hut-78 cells.

关 键 词：菝葜 Hut-78细胞 BCL-2基因 

分 类 号：R285.5[医药卫生—中药学]