激活素A与新生大鼠少突神经胶质细胞发育的相关性研究  

Experimental study on relationship between activin A and development of oligodendrocytes in neonatal rats

作　　者：唐玲[1] 安丽[1] 韩利章[2] 宋堂云[1] 宁延霞[1] 

机构地区：[1]山东大学附属济南市中心医院儿科,山东济南250013 [2]山东大学附属省立医院神经外科,山东济南250021

出　　处：《中国儿童保健杂志》2010年第8期664-667,共4页Chinese Journal of Child Health Care

基　　金：国家人力资源与社会保障留学人员科技活动择优资助项目(2009-416号)

摘　　要：【目的】探讨外源性激活素A(activin A,ACT A)对新生大鼠脑白质中少突神经胶质细胞(oligodendro-cyte,OL)发育的影响。【方法】将96只新生SD大鼠按日龄分为四组:3日龄(postnatal 3,P3),7日龄(postnatal 7,P7),14日龄(postnatal 14,P14),21日龄(postnatal 21,P21),各日龄内随机分为实验组与对照组。实验组出生后立即给予腹腔注射ACT A5 mL/kg(浓度为0.025μg/mL),对照组给予等体积的磷酸盐缓冲溶液。给药后2.5 h采集标本,光镜下观察脑组织病理学改变,电镜下观察脑组织超微结构的变化,免疫组织化学法检测脑组织中ACT A的表达。【结果】光镜下与电镜下结果一致,随日龄的增加对照组大鼠脑白质中OL逐渐发育成熟并出现髓鞘;同日龄实验组大鼠脑白质中OL较对照组增大且结构趋于成熟,日龄越小差别越明显。免疫组织化学结果显示:实验组与对照组ACT A阳性细胞计数均随着日龄的增加而减少,日龄和阳性细胞计数呈负相关(r=0.827和0.779,P均<0.001);同日龄实验组ACT A阳性细胞计数较对照组少,P3、P7、P14两组比较均有统计学意义(P<0.001 or<0.05)。P21实验组与对照组之间ACT A阳性细胞计数均值比较差异无统计学意义(P>0.05)。【结论】外源性ACT A可促进发育中的OL的成熟以及髓鞘的形成,对早期发育阶段的作用尤为显著。提示外源性ACT A早期干预对新生儿,尤其是早产儿神经系统的发育及损伤修复具有促进作用。【Objective】 To investigate the effect of exogenous activin A（ACT A） on the development of oligodendrocytes（OL） in neonatal rats. 【Methods】 96 neonatal SD rats were divided into 4 groups according to postnatal days：3,7,14 and 21-day-old.Each group was divided randomly into experimental group and control group.The rats in experimental groups were given ACT A by intraperitoneal injection in the dose of 5 mL/kg（0.025 μg/mL）after birth,and the rats in control groups were given the isovolumic phosphate buffered solution（PBS）.The specimens were collected at 2.5h after administration.The light microscope was used to observe the pathologic change and the electron microscope was used to observe the ultrastructural changes of brain tissue.The immunohistochemical method was used to detect the expression of ACT A in brain tissue. 【Results】 Under the light microscope,the OLs maturated and myelin sheath showed up gradually by the increase of postnatal days in brain white matter of control groups.The OLs of experimental groups were bigger and maturer than those of control groups of the same postnatal days,especially in groups of earlier postnatal days.The ultrastructural observation under the electron microscope showed the furthe confirmation to the results above.The result of immunochistochemistry showed,there＇s a negative correlation between the counts of positive ACT A cells and its postnatal in both experimental and control groups（r=-0.827,-0.779,P0.001）.The counts of positive ACT A cells of experimental groups were less than those of control groups at the same postnatal days in P3,P7,P14（P0.001 or0.05）,and no significant difference in P21（P0.05）. 【Conclusion】 Exogenous ACT A can promote the maturity of the developing OLs and the formation of myelin sheath,especially in the early developmental stage.

关 键 词：激活素A 新生大鼠 少突神经胶质细胞 发育 免疫组织化学 

分 类 号：R651[医药卫生—外科学]