丹参酮ⅡA抗乳腺癌细胞增殖作用研究  

Research on the inhibitory effect of Tanshinone ⅡA on breast cancer cell proliferation

作　　者：赵丕文[1] 牛建昭[1] 王继峰[1] 郝庆秀[1] 于杰[1] 孙丽萍[1] 黄春芳[1] 陶仕英[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国药理学通报》2010年第7期903-906,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30772849);高等学校创新引智计划(NoB07007);教育部博士点基金资助项目(No20060026019);国家理科基础科学研究和教学人才培养基地资助项目(No20070111)

摘　　要：目的利用雌激素受体(estrogenic receptor,ER)阳性乳腺癌T47D细胞和ER阴性乳腺癌MDA-MB-231细胞观察丹参酮ⅡA(TanshinoneⅡA)对细胞增殖活性的影响及其对雌激素受体亚型的调节功能。方法以ER拮抗剂ICI182,780为工具药,采用MTT细胞增殖实验观察1×10-6mol.L-1和1×10-7mol.L-1丹参酮ⅡA对T47D和MDA-MB-231细胞增殖的影响。利用实时荧光定量PCR法及流式细胞术检测其对T47D细胞ERα和ERβmRNA及蛋白表达情况的影响。结果丹参酮ⅡA能够抑制T47D细胞增殖,且该作用可被ICI182,780部分拮抗;对MDA-MB-231细胞增殖的抑制作用较其对T47D细胞的作用更为明显。丹参酮ⅡA可使T47D细胞ERα和ERβmRNA和蛋白表达明显增加,并使ERα/ERβ比值有所上升。结论丹参酮ⅡA具有抑制乳腺癌细胞增殖的作用,抑制强度与对其ER亚型的调节作用相关。Aim To study the effect of Tanshinone ⅡA on cell proliferation in estrogen receptor（ER）positive T47D and ER negative MDA-MB-231 breast cancer cell lines,and to explore its action on expression of estrogen receptor subtypes.Methods The proliferation rate of T47D and MDA-MB-231 cells influenced by 1×10-6 mol·L-1 and 1×10-7 mol·L-1 Tanshinone ⅡA was analyzed by MTT assay.Estrogen receptor antagonist ICI182,780 was employed as a tool.Level of ERα and ERβ mRNA in T47D cells was quantified by Real-time RT-PCR assay.Expression of ERα and ERβ protein was measured by flow cytometry.Results The proliferation rates of T47D cells treated with Tanshinone ⅡA decreased significantly.Such effects could be partly blocked by ICI182,780.Meanwhile,the proliferation rates of MDA-MB-231 cells treated with Tanshinone ⅡA decreased much more dramatically.Real-time RT-PCR and flow cytometry results showed that Tanshinone ⅡA could induce elevation of ERα and ERβ,especially ERα mRNA and protein expression level in T47D cells.Conclusions Tanshinone ⅡA shows inhibitory effects on proliferation of breast cancer cell lines.Such effects have close relationship with their regulative function on estrogen receptor subtypes in target cells.

关 键 词：丹参酮ⅡA 乳腺癌 T47D MDA-MB-231 细胞增殖 雌激素受体亚型 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]