二氮嗪对氧糖剥夺后PC12细胞凋亡及对Bcl-2蛋白的影响  

Effects of diazoxide on expression of Bcl-2 following oxygen-glucose deprivation in PC12 cell

作　　者：王占强[1] 贾春红[1] 鲁杨[1] 赵丹阳[1] 王全才[2] 张鸿[1] 

机构地区：[1]中国医科大学附属盛京医院神经内科,辽宁沈阳110004 [2]中国医科大学附属盛京医院神经外科,辽宁沈阳110004

出　　处：《中国药理学通报》2010年第7期930-933,共4页Chinese Pharmacological Bulletin

基　　金：辽宁省科学技术计划资助项目(No2008225022)

摘　　要：目的探讨二氮嗪对氧糖剥夺(OGD)PC12细胞凋亡的保护作用及其机制。方法体外培养PC12细胞株,以OGD、二氮嗪、5-羟葵酸(5-HD)处理,分为A组(正常对照组),B组(氧糖剥夺组),C组(氧糖剥夺+二氮嗪组),D组(氧糖剥夺+二氮嗪组+5-HD组),采用Annexin V-FITC/PI双染流式细胞分析仪检测凋亡率,应用免疫荧光染色和Western blot检测Bcl-2蛋白表达水平,观察二氮嗪对氧糖剥夺PC12细胞凋亡的保护作用。结果氧糖剥夺后B,C,D组PC12细胞凋亡细胞数增加,C组与B、D组比较差异均有显著性(P<0.01)。B,C,D组Bcl-2蛋白表达增加,C组达到高峰。C组与A、B、D组比较差异均有显著性(P<0.01)。结论二氮嗪能抑制氧糖剥夺PC12细胞凋亡,这一作用机制可能是增加Bcl-2表达来实现其保护作用的。Aim To study the effect of diazoxide on the apoptosis of PC12 cells induced by oxygen-glucose deprivation（OGD）and expression of Bcl-2 protein.Methods Cultured PC12 cells,treated with OGD,diazoxide and 5-HD,were divided into A（control group）,B（OGD group）,C（OGD＋diazoxide group）and D（OGD＋diazoxide＋5-HD group）.Neuronal apoptosis was detected by Annexin V-FITC/PI double-dyed flow cytometry,and the expression of Bcl-2 protein was detected by immunofluorescence and Western blot.Results The number of apoptotic PC12 cells increased after OGD in B,C,D group.C group and B,D groups were significantly different（P〈0.01）.The expression of Bcl-2 protein in B,C and D group was higher than that of A group,and that in C group was significant higher than that of A,B and D group（P〈0.01）.Conclusion Diazoxide has a protective effect on the apoptosis of PC12 cells induced by OGD,which might be correlated with the up-regulated expression of Bcl-2 protein.

关 键 词：PC12 ATP敏感性钾通道 凋亡 Bcl-2 二氮嗪 5羟-葵酸 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R743.330.22[医药卫生—基础医学]