趋化因子受体4与血管内皮生长因子在兔动脉粥样硬化斑块演进中的表达变化及意义  

Role of chemokine receptor CXCR4 in atherosclerosis angiogenesis and its possible mechanism in rabbit model of atherosclerotic plaques

作　　者：刘宏[1] 高云华[1] 夏红梅[1] 

机构地区：[1]第三军医大学新桥医院超声科,重庆400037

出　　处：《第三军医大学学报》2010年第16期1694-1697,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30772059)~~

摘　　要：目的检测兔动脉粥样斑块中趋化因子受体4(chemokine receptor4,CXCR4)蛋白表达与血管内皮生长因子(vascular endothelial growth factor receptor,VEGF)mRNA表达变化及其相关性分析,探讨CXCR4在粥样斑块血管生成中的作用及可能机制。方法 15只新西兰大白兔高脂饲料喂养+球囊拉伤腹主动脉建立动脉粥样硬化模型,分别于4、8、12周末处死动物,截取动脉血管,采用Western blot法检测CXCR4蛋白含量变化;实时荧光定量PCR检测VEGF mRNA表达变化,对两者结果进行相关性统计学分析;免疫组织化学法检测动脉粥样硬化斑块CXCR4、CD34表达与定位。结果第4、8、12周3个时相点动脉血管CXCR4蛋白含量分别为(0.46±0.03)、(0.58±0.05)、(0.65±0.07),VEGFmRNA表达分别为(1.04±0.24)、(1.99±0.21)、(3.97±0.75),两者之间呈显著正相关(Pearson相关系数r=0.869,P<0.05)。免疫组织化学CXCR4染色在血管内膜及斑块内部可见多处阳性染色;CD34染色在血管内膜斑块部位可见丰富的新生血管,棕黄色阳性表达。结论兔腹主动脉粥样硬化进程中,CXCR4和VEGF表达含量呈正相关,可能在动脉粥样硬化斑块的血管生成中具有重要作用。Objective To investigate the expressions of vascular endothelial growth factor receptor （VEGF） and chemokine receptor 4 （CXCR4） and their relationship in rabbit model of atherosclerotic plaques in order to explore the role of CXCR4 in atherosclerosis angiogenesis and its possible mechanism.Methods The 15 rabbit model of atherosclerotic plaques was established through aortic balloon injury and high cholesterol diet.Levels of CXCR4 protein and VEGF gene was detected by Western blotting and fluorescent quantitative PCR（FQ-PCR）,respectively at 4,8 and 12 weeks,and the results were analyzed statistically.Immunohistochemical staining was used to detect the expression and location of atherosclerotic plaque CXCR4 and CD34.Results At the 3 time points,the level of CXCR4 was 0.46±0.03,0.58±0.05,0.65±0.07,and the level of VEGF mRNA was 1.04±0.24,1.99±0.21,3.97±0.75,respectively.The level of CXCR4 was positively correlated with that of VEGF（correlation coefficient r=0.869,P0.05）.Immunohistochemical staining showed that CXCR4 was expressed in the vascular intima and plaque.CD34-positive staining was seen in vascular endothelial cells.There were rich new blood vessels in arterial intima plaque with CD34 expression.Conclusion The expression of CXCR4 is positively related with that of VEGF in the plaque progression,suggesting it may play an important role in angiogenesis.

关 键 词：趋化因子受体4 血管内皮生长因子 动脉粥样硬化斑块 兔 

分 类 号：R543.5[医药卫生—心血管疾病] R341[医药卫生—内科学]