非生物胁迫下水稻OsMATE基因表达分析  

Expression Analysis of OsMATE in Rice under Abiotic Stresses

作　　者：张建军[1] 胥华伟[2] 周晓垂[1] 陈建东[1] 王玉琪[1] 彭新湘[1] 

机构地区：[1]华南农业大学生命科学学院,广州510642 [2]河南科技大学农学院,河南洛阳471003

出　　处：《热带亚热带植物学报》2010年第4期435-439,共5页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(30700052);广东省自然科学基金博士启动项目(0700680);华南农业大学校长基金项目(4600-K06332);河南科技大学博士启动基金项目(09001271)资助

摘　　要：从水稻(Oryza sativa L.)叶片中克隆到与高粱(Sorghum bicolor)SbMATE最相似的同源基因OsMATE,利用半定量RT-PCR分析了OsMATE在水稻不同组织、不同非生物胁迫以及抗铝和铝敏感水稻品种中的表达。结果表明,OsMATE在水稻根和叶中表达非常低,叶鞘中几乎没有表达;铝、镉、砷、盐、铁、PEG6000、百草枯和ABA等非生物胁迫都可以诱导水稻根OsMATE的大量表达,而在水稻叶中,只有砷、盐和PEG6000可以少量诱导OsMATE的表达;并且铝毒胁迫下抗铝品种根中OsMATE的表达明显高于铝敏感品种。这说明非生物胁迫下OsMATE在水稻抗逆中可能发挥重要作用。OsMATE, the closest homology of sorghum （Sorghum bicolor） SbMATE, was cloned by PCR technique from rice （Oryza sativa） leaves, and then its expression pattern was analysed in different tissues and under abiotie stresses by semi-quantitative RT-PCR. The result showed that OsMATE expressed lowly in rice roots and leaves, and almost none in leaf sheaths. OsMATE expressed strongly in rice roots induced by abiotic stresses, such as aluminum （Al）, cadmium, arsenic, salt, iron, PEG6000, paraquat and ABA, while little expressed in leaves by arsenic, salt and PEG6000 stress. The expression of OsMATE in roots of the Al-resistant ＇XN1 ＇ was more than that in the Al-susceptible ＇ XX2＇ under A1 stress. It suggested that OsMATE could play an important role in stress resistance of rice under abiotic stresses.

关 键 词：水稻 OSMATE 半定量RT—PCR 非生物胁迫 

分 类 号：S511[农业科学—作物学]