鸡传染性支气管炎病毒地方分离株S1、N和M基因的克隆与表达  

Cloning and Expression of the S1,N and M Gene of IBV

作　　者：闫芳[1] 赵宇军[1] 吴倩[1] 岳文斌[1] 李绪英[1] 吉文汇[1] 刘娟[1] 刘风波[1] 任家琰[1] 化丽珍[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国农学通报》2010年第16期14-17,共4页Chinese Agricultural Science Bulletin

基　　金：山西省自然科学基金"IBV山西变异株基因型调查及其S;M和N基因试验免疫研究"(20070110830);山西高校科技研究开发项"山西变异型鸡传染性支气管炎灭活疫苗的开发研究"(200611014);山西农业大学博士后基金"IBV山西变异株基因型调查"

摘　　要：为研究鸡传染性支气管炎病毒DNA疫苗,构建了表达IBVS1、M和N基因真核表达载体,并且BHK-21细胞和小鼠体内进行了表达检测,将IBVSX16株的S1、N和M基因克隆入真核表达载体pVAX1,构建IBV真核表达载体pVAX1-16S1、pVAX1-16N和pVAX1-16M。体外转染BHK-21细胞,用间接ELISA检测到目的蛋白表达,用构建的3个表达载体免疫小鼠,从小鼠血清中检测到了抗IBV抗体,初步证实用IBVS1、N和M基因作为核酸疫苗免疫动物,可以激活机体免疫应答。For efficacious DNA vaccine development against infectious bronchitis virus（IBV）,we constructed plasmids containing S1,N and M protein of virulent IBV.These plasmids were tested by transient transfection assays in BHK-21 cells and in mice.The S1,N and M genes of IBVSX16 strain were sub-cloned into the eukaryotic expression plasmid pVAX1,which is driven by CMV promoter,named pVAX1-16S1,pVAX1-16N and pVAX1-16M,respectively.Purified plasmids DNA were transfected into BHK-21 cells by lipofectamine.The IBV S1,N and M proteins were expressed in BHK-21,which do specifically react to the CDV antisera.The mice inoculated with these plasmids induced the immune response against IBV.

关 键 词：鸡传染性支气管炎病毒 S1基因 N基因 M基因 

分 类 号：S885.3[农业科学—特种经济动物饲养]