3-氨基喹啉与常用基体在MALDI-TOFMS测定天然提取植物多糖的比较  

Comparison of 3-Aminoquinoline and Common Matrixes for MALDI-TOF MS Analysis of Naturally Separated Oligosaccharide

作　　者：周丽华[1] 邓慧敏[2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]中山大学分析测试中心,广东广州510275

出　　处：《广东化工》2010年第8期261-262,243,共3页Guangdong Chemical Industry

基　　金：广东省自然科学基金(990209);广东工业大学博士启动基金(073009)

摘　　要：采用3-氨基喹啉(3-AQ)、二元基体3-AQ/2,5-二羟基苯甲酸(DHB)及常用于糖类物质测定的DHB和二元基体DHB/1-羟基异喹啉(1-HIQ)对一天然提取植物糖样进行了基体辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)分析。实验结果表明:此植物糖样由线形和环形聚己糖组成,其最大分子量至少达3000 Da;3-AQ与常用基体DHB及DHB/HIQ相比较,能提供更准确、更全面的样品组成及分子结构信息,且具有优越于常用基体解吸电离环状糖分子的能力。3-aminoquinoline（3-AQ）,3-AQ/2,5-dihydroxybenzoic acid（DHB）,DHB and DHB/1-hydroxyisoquinoline（1-HIQ） were used as matrices for matrix-assisted laser desorption/ionization time-of –flight mass spectrometric（MALDI –TOF MS） analysis of a naturally separated oligosaccharide.The results showed that the oligosaccharide sample was composed of cyclic and linear chain hexosans with a molecular weight at least up to 3000 Da.More components and structural information of the oligosaccharide can be acquired from the spectra with 3-AQ as matrix than the commonly used matrices,DHB and DHB/1-HIQ.With the increasing concentration of 3-AQ in the binary matrix of 3-AQ/DHB,the peaks of the cyclic chains in the oligosaccharide can be readily observed,demonstrating that the cyclic chain can be more easily desorbed and ionized by 3-AQ than by DHB and DHB/1-HIQ.

关 键 词：3-氨基喹啉 基体 基体辅助激光解吸/电离飞行时间质谱 天然多糖 

分 类 号：O65[理学—分析化学]