苹果果实发酵液对草莓防御酶系活性及抗病性的影响  

Influences of Apple Fruit Broth on Activities of Defense Enzymes and Disease Resistance on Strawberry

作　　者：王荣娟[1] 戚亚平[1] 宋备舟[1] 黄彬[2] 王雷[2] 姚允聪[1] 姬谦龙[2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物技术系,北京102206

出　　处：《中国农学通报》2010年第16期250-255,共6页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑重大项目"主要果树高效标准生产技术"(2008BAD92B08);北京市农委资助项目(20070624);北京农学院基础科学基金资助项目(062ZD2JW205)

摘　　要：探讨在有机栽培方式下,苹果果实发酵液诱导草莓抗灰霉病(Botrytis cinerea)的作用。用4个处理喷施草莓,5天后接种灰霉病病菌。接菌6天后统计不同处理的病斑数,同时测定过氧化物酶(POD)、多酚氧化酶(PPO)及β-1,3-葡聚糖酶在接种后12天内的动态变化。Tr2处理后,草莓的抗病指数达到34.6%,显著高于对照,其他处理差异不显著。喷施Tr1、Tr2、Tr3可使POD、PPO酶活性高于对照,β-1,3-葡聚糖酶活性高峰出现较对照早。苹果果实发酵液100倍稀释液和0.02mol/mL水杨酸混合喷施可诱导草莓对灰霉病产生抗性。这对草莓有机生产中草莓灰霉病的防治提供了新途径。This paper explored the effect of the apple fruit broth on inducing resistance of strawberries to Botrytis cinerea under the organic cultivation.We sprayed strawberries with four different treatments.Then 5 days later,the strawberries were inoculated with Botrytis cinerea.6 days after inoculating,the numbers of necrotic lesions were counted and activities of peroxidase,polyphenol oxidase and β-1,3-glucanase were assaied.The results showed that the Tr2 could not only induce resistance of the strawberries on the Botrytis cinerea,its index of disease resistance reached 34.6%,which was significantly higher than the control.Difference between other treatments was not significant.Sprayed with Tr1,Tr2,Tr3,the activities of POD,PPO were higher than the control,activity of β-1,3-glucanase appeared earlier than the control did.The mixed liquor of 100-fold diluted apple fruit broth and 0.02mol/mL salicylic acid could induce desease resistance of the strawberry to Botrytis cinerea.The research may provide a new mathod to prevent the desease on strawberry.

关 键 词：苹果果实发酵液 草莓 灰霉病 水杨酸 抗病性 

分 类 号：S432.4[农业科学—植物病理学]