PR8流感病毒突变株的制备及其在鸡胚上生长特性的研究  

Development of influenza virus PR8 mutants and their characteristics on embryonated chicken eggs

作　　者：王朝霞[1,2] 滕巧泱[2] 戴晓光[2] 黄海碧[2] 张树梅[2] 张旭[2] 徐大伟[2] 李培锋[1] 李泽君[2] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]中国农业科学院上海兽医研究所农业部寄生虫学重点开放实验室,上海200241

出　　处：《中国兽医科学》2010年第8期788-792,共5页Chinese Veterinary Science

基　　金：上海市浦江人才计划项目(09PJ1411900);转基因生物新品种培育重大专项(2009ZX08010-022B)

摘　　要：在前期发现流感病毒WSN株的NS2或NP蛋白突变使病毒在细胞上增殖能力明显提高的基础上,研究了这些突变是否会使PR8病毒效价在鸡胚上进一步提高的可行性,通过反向遗传操作技术获得了NS2E67S和NPP277A两株PR8突变病毒。经过比较突变病毒与野生病毒在鸡胚中的增殖能力,发现NS2E67S突变病毒在鸡胚上增殖后的效价与HA抗原表达量均明显提高,而NPP277A突变病毒在鸡胚上的增殖能力不及野生PR8病毒。该研究结果将对进一步研究病毒基因变异与宿主互作机制奠定基础,有可能通过NS2E67S突变病毒对改良流感疫苗具有重要的应用价值。In previous studies,we found that some A/WS/33（WSN） mutants on NS2 and NP proteins showed increasing replication abilities on MDCK cells.In order to characterize the amplification of PR8 mutants on these proteins in embryonated chicken eggs,the mutants were obtained by PCR-mutation and influenza virus reverse genetic technology.After being replicated in chicken embryonated eggs,the viral titer and HA titer of mutant NS2 E67S were higher than that of wt PR8,while these titers of NP P277A were lower than that of wt PR8.These results filled gap of knowledge on the balances of viral gene mutation and viral adaption to the host,indicating that mutant NS2 E67S would be very important in influenza vaccine development.

关 键 词：流感病毒 PR8 突变株 

分 类 号：S852.659.5[农业科学—基础兽医学]