贫困山区妊娠妇女HCMV感染与血清IL-8的关系  

Relationship of HCMV infection and serum level of IL-8 of pregnant women in Qinba mountainous areas

作　　者：宋晖[1] 李芬[1] 许静[1] 张欣文[1] HUANG Hui 

机构地区：[1]西安交通大学医学院第一附属医院妇幼保健中心,陕西西安710061 [2]马里兰大学数学与统计学院,马里兰州巴尔的摩市21250

出　　处：《中国妇幼健康研究》2010年第4期528-530,共3页Chinese Journal of Woman and Child Health Research

摘　　要：目的 研究贫困山区孕期人巨细胞病毒感染状况,探讨苓与白细胞介素8 （Interleukin-8,IL-8）的关系.方法 用ELISA和PCR方法 检测贫困山区323例正常孕妇外周血特异性 HCMV IgM、IgG抗体及HCMV DNA,对其中27例孕期HCMV 活动性感染者及48例孕期无HCMV活动性感染者进行随访,检测产后乳汁及新生儿尿液HCMV DNA;并采用双抗体夹心ELISA法检测孕妇及乳母外周血中IL-8水平.结果 孕妇HCMV活动性感染率为8.36%,宫内传播率为25.93%,孕期CMV活动性感染者及宫内传播者其外周血IL-8水平显著高于未感染者（P〈0.05）,乳汁排毒者和未排毒者间血清IL-8水平没有明显变化.结论 近年来贫困山区孕期HCMV活动性感染率有所下降,但较国内外报道仍高;IL-8在孕期活动性HCMV感染及宫内感染中可能起着重要的免疫调节作用.Objective To investigate infection situation among human cytomegalovirus （HCMV） among pregnant women in poor Qinba mountainous areas, and to study relationship beween interleukin-8 and HCMV infection. Methods PCR and ELISA were used to detect specific HCMV IgM, IgG antibodies and HCMV DNA in peripheral blood of 323 normal pregnant women in poor Qinba mountainous areas, of which 27 pregnant women with active HCMV infection during pregnancy and 48 pregnant women without active HCMV infection during pregnancy were followed up and HCMV DNA in breast milk of mother and urine of the neonate were detected, and double-antibody sandwich ELISA was used to detected serum level of IL-8 in peripheral blood of pregnant women and breasffeeding mothers. Results The active HCMV infection rate among pregnant women was 8.36% , and the intrauterine vertical transmission rate of HCMV was 25.93%, the serum level of IL-8 of the pregnant women with active HCMV infection during pregnancy and the intrauterine disseminators of HCMV were significantly higher than those of pregnant women without HCMV infection （ P 〈 0.05 ）, and between pregnant women who had positive HCMV-DNA in their breast milk and those pregnant women who hadn＇ t positive HCMV-DNA in their breast milk, there was no significant difference in serum IL-8 level. Conclusion In recent years, the active HCMV infection rate during pregnancy among pregnant women in poor Qinba mountainous areas has declined, but it is still higher than domestic and foreign reports. IL-8 may play an important role in immune regulation in active HCMV infection and intrauterine transmission of HCMV.

关 键 词：巨细胞病毒 妊娠 宫内感染 白细胞介素-8 

分 类 号：R71[医药卫生—妇产科学]