平喘I号对呼吸道合胞病毒哮喘小鼠TNF-a ET-1影响的研究  

Study on PingchuanⅠfor the Effect of TNF-a ET-1 of Asthmatic Mice Infected by Respiratory Syncytia Virus

作　　者：杨季国[1] 张春玲[1] 徐燕立[1] 程东庆[1] 林之涵[1] 林馨[1] 

机构地区：[1]浙江中医药大学附属第三医院,浙江杭州310009

出　　处：《中华中医药学刊》2010年第8期1674-1675,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y2080247);浙江省中医药科技计划项目(2008CA032)

摘　　要：目的:观察平喘Ⅰ号对呼吸道合胞病毒(RSV)感染引起BALB/c小鼠哮喘模型血清中肿瘤坏死因子-a(TNF-a)、内皮素-1(ET-1)含量的影响,探讨其治疗哮喘发作的作用机制。方法:72只BALB/c小鼠随机分成6组,随机分为正常对照组、模型对照组、地塞米松组、平喘Ι号低、中、高剂量组,每组12只。予RSV致敏,除正常对照组和模型对照组用等量生理盐水外,其余各组用相应药物干预。末次激发48h后取血清采用ELISA法测定各组TNF-a和ET-1含量。结果:与正常对照组比较,哮喘模型组小鼠血清TNF-a、ET-1含量明显增高(P<0.01)。与模型对照组比较,平喘I号各剂量组和地塞米松组均可降低哮喘小鼠血清中TNF-a和ET-1水平(P<0.01或P<0.05),其中平喘I号高剂量组与地塞米松组水平接近。结论:平喘Ⅰ号能降低哮喘小鼠血清TNF-a、ET-1含量,并具有量效关系。由此推测,通过降低血清中TNF-a和ET-1水平,从而减轻气道炎症反应和气道组织的重构,是平喘Ⅰ号治疗哮喘的作用机制之一。Objective：To observe the Pingchuan I on respiratory syncytial virus （RSV） infection in the BALB/c mouse asthma model of serum tumor necrosis factor-a（TNF-a）,endothelin-1（ET-1） levels,and to explore the therapeutic mechanism of asthma attacks.Methods：72 BALB/c mice were randomly divided into six groups,which were normal control group,model control group,dexamethasone group,Pingchuan I low,middle and high-dose group,12 rats in each.Sensitized to the RSV,in addition to the normal control group and model control group received normal saline,all the other groups with the corresponding drug intervention.48h after the last challenge,using ELISA to detect the serum TNF-a and the ET-1 levels in each group.Results：Compared with normal control,asthma model groups＇ serum TNF-a,ET-1 were significantly higher（P〈0.01）.With the model control group,Pingchuan I each dose group and dexamethasone group can reduce asthma in mice serum TNF-a and ET-1 levels（P〈0.01 or P〈0.05）,in which,the high dose group and dexamethasone group close to.Conclusion：The Pingchuan I can reduce asthma serum TNF-a,ET-1 levels,and has dose-effect relationship.The results suggested that,by lowering serum TNF-a and ET-1 levels,thereby reducing airway inflammation and airway remodeling organization is the mechanism of Pingchuan I in treating asthma.

关 键 词：呼吸道合胞病毒 支气管哮喘 平喘Ⅰ号 TNF-A ET-1 

分 类 号：R285.5[医药卫生—中药学]