不同产地中药材枸骨叶中总皂苷的含量测定  

Content determination of total saponins in Folium ilicis cornutae from different area

作　　者：周国莉 舒柯[2] 聂骊晓[2] 任守利[2] 刘宇婧[2] 刘塔斯[2] 

机构地区：[1]深圳罗湖区中医院,广东深圳518001 [2]湖南中医药大学药学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2010年第7期33-35,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科技资源共享平台项目(2005DKA21000);湖南省教育厅十一五中药学重点学科资助项目

摘　　要：目的研究不同产地枸骨叶中总皂苷的含量。方法用5%香草醛-冰醋酸及高氯酸显色,在波长548nm处用比色法测定了枸骨叶中的总皂苷含量。结果回归方程为Y=0.0180X+0.0961,相关系数r=0.9991。湖南资兴、永兴两县所产枸骨叶的总皂苷含量高,分别为1.437%、1.383%。结论本方法操作简便、可行、重复性好。Objective To study the content of total saponins in leaf of Ilex cornuta from differentarea.Method Coloration was used with 5% vanillin acetum and perchloric acid,the content of triterpenoid saponins in Folium Ilicis cornutae was determined at 548 nm.Result The linear regression equation was Y=0.018 0X ＋0.096 1,r =0.999 1.The content of total saponins in folium ilicis cornutae produced in ZiXing、Yongxing of Hunan was more higher,1.437% and 1.383% respectively.Conclusion The method is simple,reproducible and practicable for the determination of triterpenoid saponin.

关 键 词：枸骨叶 熊果酸 分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学]