柯萨奇-腺病毒受体基因-968G/A多态性与柯萨奇病毒性心肌炎的关系  

Relationship between polymorphism of Coxsackie-adenovirus receptor gene-968G /A and Coxsackie viral myocarditis

作　　者：杨云华[1] 邓晖[1] 刘丹莉[1] 杜九中[1] 李双杰[2] 

机构地区：[1]南华大学第一附属医院儿科,湖南衡阳421001 [2]湖南省儿童医院感染科,湖南长沙410000

出　　处：《临床儿科杂志》2010年第8期755-757,共3页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30271665);衡阳市科技局研究资助项目(No.2005KS01-024)

摘　　要：目的探讨柯萨奇-腺病毒受体(CAR)基因-968G/A多态性与中国南方汉族人群柯萨奇病毒性心肌炎的关系。方法收集病毒性心肌炎患儿280例,将其中CVB-IgM阳性130例作为心肌炎组,并选择同期130名健康儿童作为对照组,采用聚和酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)、DNA测序等方法检测2组CAR基因-968G/A多态性,采用酶联免疫吸附法(ELISA)测定两组血清CAR水平。结果心肌炎组GG基因型和G等位基因频率均显著高于对照组(P<0.05)。心肌炎组血清CAR水平明显高于对照组(P<0.01),CAR基因-968G/A多态位点中含G等位基因(GG+GA)者血清CAR水平和CVB感染率均显著高于非G等位基因(AA)携带者(P均<0.05)。结论 CAR基因-968G/A多态性可能与中国南方汉族人群柯萨奇病毒性心肌炎有关,G等位基因是柯萨奇病毒性心肌炎的易感基因。Objectives To investigate the relationship between polymorphism of Coxsackie-adenovirus receptor（CAR） gene G968A and Coxsackie viral myocarditis in Han Population of South China.Methods 130 Coxsackie virus B-IgM positive patients（myocarditis group） derived from 280 patients with viral myocarditis and 130 healthy children（control group） were enrolled.The 968G /A polymorphism in CAR gene was detected by polymerase chain reactionrestriction fragment length polymorphism and DNA sequencing in two groups.Serum CAR levels in the two groups were determined by enzyme-linked immunosorbent assay.Results The frequencies of CAR GG genotypes and G alleles in myocarditis group were significantly higher than those in control group（P〈0.05）.Serum CAR level in myocarditis group was significantly higher than that in control group（P〈0.01）.The people with CAR 968G allele（GG ＋ GA） had higher level of serum CAR and Coxackie virus B infection rate than those only with CAR 968A allele（AA）（P〈0.05）.Conclusions CAR gene-968G /A polymorphism is associated with Coxsackie viral myocarditis in the Han population of South China,and G allele is a susceptible gene of Coxsackie viral myocarditis.

关 键 词：柯萨奇-腺病毒受体 基因多态性 病毒性心肌炎 

分 类 号：R725.4[医药卫生—儿科]