模拟失重状态下大鼠心血管中枢组织基因组变化研究  

Research on the Gene Changes of Rat Cardiovascular Central Tissue under Simulated Weightlessness

作　　者：刘军莲[1] 李勇枝[1] 杨兴胜[1] 盖宇清[1] 高建义[1] 谢琼[1] 陈章煌[1] 刘建中[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《航天医学与医学工程》2010年第4期235-238,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金项目(30772694);中华人民共和国科技部国际合作项目(2006DFA32250);中国载人航天项目(010202)

摘　　要：目的拟通过动物模拟失重模型,研究模拟失重条件下心血管调节功能改变发生的分子机制。方法利用基因芯片技术,检测5例模拟失重大鼠和5例对照大鼠心血管中枢组织基因芯片的变化情况,从基因层面上揭示模拟失重心血管功能变化的实质。结果基因芯片结果显示:模拟失重状态导致心血管中枢部位ADP核糖基化因子样2(ADP-ribosylation factor-like2,Arl2)、孕酮和adipoQ受体家族成员Ⅳ(progestin and adipoQ receptor family member Ⅳ,paqr4)、丙酮酸脱氢酶激酶同工酶1(pyruvate dehydrogenasekinase,isozyme1,pdk1);促生长素抑制蛋白(somatostatin,sst)、跨膜蛋白酶(transmembraneprotease、tmprss5)、丝氨酸5(脊突蛋白)serine5(spinesin)发生了改变。结论失重状态下,心血管功能的变化可能与Arl2、Paqr4、Pdk1、Sst、Tmprss5涉及的转导通路的改变有关。Objective To study the molecular mechanism of cardiovascular regulatory functional changes in rats under simulated weightlessness condition. Methods The gene changes of cardiovascular central tissue in five control rats and the five models were examined with gene chip technology to display the essence of cardiovascular functional changes. Results The gene chips showed that ADP-ribosylation factor-like2,progestin and adipoQ receptor family member IV,pyruvate dehydrogenasekinase,isozyme1;somatostatin,sst, transmembraneprotease, tmprss5 and serine5（spinesin）were changed in the cardiovascular center under simulated weightlessness. Conclusion Under weightlessness the changes of cardiovascular function are related to the signal channels about Arl2, Paqr4, Pdk1, Sst and Tmprss.

关 键 词：失重模拟 心血管中枢 基因芯片 基因组 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]