亚急性汞中毒大鼠脊髓及背根神经节TNF-α表达与药物干预研究  

TNF-α Expression in Spinal Cord and DRG of Sub-acute Mercurialism Rats and Study of Drug Intervention

作　　者：郁东[1] 解镭[1] 马娟[1] 赵昕[1] 刘海量[1] 周永田[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南市250002

出　　处：《职业与健康》2010年第17期1921-1923,共3页Occupation and Health

基　　金：山东省医学科学院资助项目(编号:2004-0023)

摘　　要：目的观察亚急性汞中毒大鼠脊髓和背根神经节(DRG)肿瘤坏死因子-α(TNF-α)的表达。方法雄性SD大鼠40只,体重180～220g,分为7组;对照组(10只)经口灌胃生理盐水(NS),2ml/次,1次/d;A、B、C、D、E、F组(各5只)经口灌胃HgCl2溶液,1次/d。A、B、C组为17mg/kg,D、E、F组为8.5mg/kg。7组均连续灌胃18～22d。染汞组动物出现汞中毒症状后,处死A、D组和对照组各5只大鼠。随后B、E组用二巯丙磺钠注射液(DMPS)按28mg/kg腹腔注射驱汞2个疗程(驱汞3d,休息4d为1个疗程);C组用DMPS+己酮可可碱(按29.22mg/kg经口灌胃,1次/d,共14d);F组腹腔注射NS1ml/次,方法同DMPS。实验结束后处死全部动物,灌注固定后,取出脊髓L5-6节及L5、L6两侧DRG并制成病理切片。用免疫组化SABC法测定DRG和脊髓TNF-α表达水平。结果染毒组大鼠脊髓和DRG内TNF-α平均灰度均显著低于对照组;阳性反应物平均面积均显著高于对照组(P<0.05);B、E组用DMPS驱汞2个疗程及F组腹腔注射NS2个周期后均未见TNF-α表达显著减弱,C组(DMPS+已酮可可碱)脊髓和DRG内TNF-α平均灰度显著提高,平均面积减少(P<0.05)。结论亚急性HgCl2中毒大鼠脊髓和DRG内TNF-α表达显著增强,SMPS联合已酮可可碱能够有效抵制TNF-α表达。[Objective]To investigate the express of tumor necrosis factor-α（TNF-α） in spinal cord and dorsal root ganglion（DRG） of sub-acute mercurialism rats.[Methods]Fourty SD rats（mal,weighting 180～220 g） were divided into 7 groups.Control group （10 rats） received normal saline （NS） 2 ml by lavage 1 time daily.Group A,B,C（5 rats,respectively） received HgCl2 solution at dose of 17 mg/kg,group D,E,F（5 rats,respectively） received HgCl2 solution at dose of 8.5 mg/kg by lavage,1 time daily.The administration by lavage lasted 18-22 days in all 7 groups.After appearing symptoms of mercury poisoning in mercury group,5 rats of control group and group A and D were dissected and the chelator （DMPS） was injected into the abdomen of alive rats of group B and E at dose of 28 mg/kg for 2 courses（removing mercury 3 days and resting 4 days as a treatment course）.Then Group C was given DMPS＋ pentoxifylline and group F was injected NS 1ml into the abdomen only （29.22mg/kg lavage,1time /day,totally 14 days）.The rats were all dissected and the spinal cord （L5-6）and the DRG of L-5 and L-6 were gotten after the trial finished.Then the pathological section was made after perfusion fixation.DRG and TNF-αexpression was detected according to immunohistochemistry SABC method.[Results]The average gray scale of TNF-α level in spinal cord and DRG of the rats exposed to HgCl2 were significantly lower than that of control group,while average area of positive reactant was significantly higher the control group （P0.05）.TNF-α expression was not significantly reduced after two 7-day cycles of group B and E using DMPS for removing mercury and group F using NS intraperitoneal injection.The average gray scale of TNF-α level of group C in spinal cord and DRG increased significantly after 2 courses using DMPS＋ pentoxifyllinem,while average area decreased （P0.05）.[Conclusion]TNF-αexpression significantly increases in spinal cord and DRG of sub-acute mercurialism rats,while it can be inhibited effect

关 键 词：汞中毒 神经病理性疼痛 肿瘤坏死因子-α 脊髓 背根神经节 

分 类 号：R114[医药卫生—卫生毒理学]