荷叶中生物碱提取及纯化工艺研究  

Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis

作　　者：朱晓月[1] 刘虹[1] 郝彧[1] 王萌[1] 张德芹[1] 

机构地区：[1]天津中医药大学中医药研究中心方剂学教育部重点实验室,天津300193

出　　处：《中国实验方剂学杂志》2010年第9期22-24,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大科技专项"重大新药创制"(2009ZX09102-128);治疗糖尿病组分中药的联合开发(2009DFA31070)

摘　　要：目的:优选荷叶总生物碱的提取纯化工艺。方法:荷叶药材经过冷浸、浓缩、索氏提取、萃取、碱析,分离荷叶总生物碱,并以酸性染料比色法测定荷叶总生物碱含量。结果:荷叶用含0.1%盐酸的70%乙醇浸泡提取36 h,提取3次后中和、浓缩,再以乙酸乙酯为溶媒索氏提取3 h,再以pH 1的酸水及乙酸乙酯先后进行萃取,所得乙酸乙酯萃取液用40%氢氧化钠溶液碱析,所制荷叶总生物碱质量分数达到90%以上。结论:此提取纯化工艺操作简便,方法可行,重复性好,为荷叶总生物碱的开发利用提供了实验依据。Objective：To study the extraction and purification technology of total alkaloids in Folium Nelumbinis.Method：The total alkaloids were obtained by impregnation,concentration,Soxhlet extraction and alkaline separation.Acid dye colorimetry was used to determine total alkaloids in Folium Nelumbinis.Result： Folium Nelumbinis was soaked in 1% HCl/70% ethanol for 36 h 3 times.After neutralization and concentration,Soxhlet extraction was carried out for 3 hours with ethyl acetate.Then the concentrate was extracted successively by acidic water at pH 1 and ethyl acetate.40% NaOH was used for alkaline separation.The content of total alkaloids was above 90%.Conclusion： This extraction and purification technology was convenient,feasible,and reproducible,that would provide basic for the research and development of Folium Nelumbinis.

关 键 词：荷叶 总生物碱 工艺研究 

分 类 号：R283.6[医药卫生—中药学]