GC法测定灵丹草油中β-芹子烯的含量  

Determination of β-selinene in Volatile Oil from Laggera pterodonta(DC. )Benth by GC

作　　者：余启荣[1] 高柏丽[1] 胡玉霞[1] 周春莺[1] 高茗 和芳[1] 

机构地区：[1]北京创立科创医药技术开发有限公司,北京100029 [2]云南盘龙云海药业有限公司,云南675000

出　　处：《中国实验方剂学杂志》2010年第9期44-45,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家十一五科技支撑计划项目(2006BAI06A18-01)

摘　　要：目的:建立灵丹草油中β-芹子烯的含量测定方法。方法:采用气相色谱法,色谱柱为HP-5MS毛细管柱;检测器为FID;程序升温为初始温度80℃,保持2 min,以每分钟2℃的速率升温至150℃,再以每分钟10℃的速率升温至200℃,保持8 min。结果:β芹子烯在0.014 76～0.590 4μg线性关系良好(r=0.999 9,n=5),平均回收率为99.38%(n=5),RSD1.26%。结论:本实验方法可靠,重复性好,专属性强,可用于灵丹草油中β-芹子烯的含量测定,以控制灵丹草油的质量。Objective：To establish a method for determination of β-selinene in Laggera pterodonta（DC.）Benth Oil by GC.Method：The GC equipped with FID detector,and a HP-5MS capillary column was used.The temperature program of column oven was set as follows： 80 ℃ for 2 min,increased to 150 ℃ at a rate of 2 ℃·min-1,then at 10 ℃·min-1 to 200℃,held for 8 min.Result： β-selinene was linear（r =0.999 9,n =5） in the range of 0.014 76 ～0.590 4 μg;The average recovery was 99.38%（n =6）,and RSD 1.26%.Conclusion： The method can be used to determine the content of β-selinene in L.pterodonta Oil.

关 键 词：灵丹草油 β-芹子烯 气相色谱法 

分 类 号：R284.1[医药卫生—中药学]