固相萃取-高效液相色谱法测定大鼠血浆中柴胡皂苷d的浓度  

Solid Phase Extraction-high Performance Liquid Chromatography Determination of Saikosaponin d in Rat Plasma

作　　者：何昱[1] 张茹萍[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《中国实验方剂学杂志》2010年第9期46-48,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省教育厅课题(20050815)

摘　　要：目的:建立用固相萃取-高效液相色谱法定量大鼠灌服柴胡水提液后血浆中柴胡皂苷d的方法。方法:大鼠灌服柴胡水提液后在不同时间点眼眶静脉取血,血浆经Waters OasisHLB固相萃取小柱纯化,洗脱液用氮气吹扫至干,流动相溶解后进样;用YMC-Pack ODS C18色谱柱以及乙腈-水为流动相的色谱体系检测其中柴胡皂苷d的含量。结果:柴胡皂苷d在1.28～81.60μg·mL-1的浓度范围内线性关系良好(相关系数r=0.997 8),定量下限为1.28μg·mL-1。相对回收率为94.6%～102.8%,日间、日内RSD均小于8%。结论:该方法灵敏、准确,可用于大鼠血浆中柴胡皂苷d的浓度测定。Objective：To develop a SPE-HPLC method for the determination of saikosaponin D in rat plasma after being administered Radix Bupleuri water extract intragastrically.Method：The blood was taken from rat orbital venous at different time points,and the plasma was purified by using the Waters Oasis solid-phase extraction column.The eluate was dried with nitrogen,and then dissolved with mobile phase.The saikosaponin D was analyzed by chromatographic system consisting of YMC-Pack ODS C18 column and acetonitrile-water as mobile phase.Result： Saikosaponin D was linear in range of 1.28-81.60 μg·mL-1（r=0.997 8）.And the quantitative limit was 1.28 μg.mL-1.Relative recovery was 94.6%-102.8%;And the within-day precision and between-day precision were less than 8%（RSD）.Conclusion： This method is sensitive,accurate,and can be used for determination of saikosaponin D in rat plasma.

关 键 词：固相萃取 高效液相 柴胡皂苷D 

分 类 号：R284.1[医药卫生—中药学]