镇肝熄风汤载药脑脊液对PC12细胞损伤的保护作用  

Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP^+

作　　者：李成冲[1,2] 李佳鸣[1,2] 王刚[1,2] 邹德佳[1,2] 耿玉涛[1,2] 李强[1] 牛英才[1] 

机构地区：[1]齐齐哈尔医学院医药科学研究所,黑龙江齐齐哈尔161042 [2]佳木斯大学化学与药学院,黑龙江省生物药制剂重点实验室,黑龙江佳木斯154007

出　　处：《中国实验方剂学杂志》2010年第9期134-137,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省自然科学基金项目(ZA2006-1);黑龙江省教育厅科学技术研究面上项目(11541413)

摘　　要：目的:探讨镇肝熄风汤载药脑脊液对甲基-苯基-吡啶离子(1-methyl-4-phenylpyridinium,MPP+)诱导的PC12细胞损伤的保护作用。方法:不同浓度的镇肝熄风汤载药脑脊液预处理体外培养的PC12细胞后,加入MPP+诱导PC12细胞损伤模型。用MTT法检测PC12细胞活力,实时定量PCR检测Bax和Bcl-2 mRNA的表达,Western blot检测Bax和Bcl-2蛋白表达。结果:MPP+处理72～96 h,与空白对照组比较,模型组细胞活力明显降低,Bcl-2 mRNA和蛋白表达显著下调,Bax mRNA和蛋白表达显著上调(P<0.05)。与模型组比较,不同浓度的镇肝熄风汤载药脑脊液细胞活力明显升高,Bcl-2 mRNA和蛋白表达显著上调,Bax mRNA和蛋白表达显著下调,差异具有统计学意义(P<0.05)。结论:镇肝熄风汤载药脑脊液对MPP+诱导的PC12细胞具有保护作用,可能是通过上调Bcl-2和下调Bax的mRNA和蛋白表达而发挥抑制PC12细胞凋亡的作用。Objective：To investigate the protective effect of cerebrospinal fluid containing Zhenganxifeng decoction（CSF-ZGXF） on pc12 cell induced by 1-methyl-4-phenylpyridinium ion（MPP ＋）.Method：Neurotoxicity was induced by addition of MPP ＋ into PC12 cell cultures.And different doses of CSF-ZGXF were administrated 24 h before addition of MPP ＋ into PC12 cell cutrure.Cell viability,mRNA and protein of Bax and Bcl-2 expression was assayed by MTT,real-time PCR and western blot.Result：Optical density for PC12 cells treated with MPP ＋ for 48-96 h was lower than that of the control group.The mRNA and protein expression of Bcl-2 was down-regulated and those of Bax was up-regulated in MPP ＋ groups compared with the control group.The mRNA and protein expression of Bcl-2 were up-regulated and those of Bax were down-regulated in CSF-ZGXF group compared with the MPP ＋ group.Conclusion：The results suggest that protective effect of CSF-ZGXF on PC12 cells may correlate with downregulation of mRNA and protein expression of Bax and up-regulation of those of Bcl-2.

关 键 词：帕金森病 镇肝熄风汤 载药脑脊液 1-甲基-4-苯基吡啶离子 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]