黄芩、柴胡及其配伍提取物体外抗柯萨奇B3m病毒及对细胞活性的影响  

Effect of Skullcap,Bupleurum chinense and Their Compatible Extract on Cell Infected by Coxsackie Virus B3m (CVB3m) in vitro

作　　者：刘芳[1] 王雪峰[1] 闫丽娟[1] 南春红[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《中国实验方剂学杂志》2010年第9期173-176,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30371832)

摘　　要：目的:观察黄芩、柴胡及其配伍提取物体外抗柯萨奇B3m病毒(CVB3m)及对细胞活性的影响。方法:采用微量细胞培养法,以细胞病变法和MTT法判定细胞活性,观察黄芩、柴胡及其配伍提取物以及黄芩两种不同提取制剂体外抗CVB3m病毒和对细胞活性的影响,确定各组药物的抑制病毒指数和药物对细胞的预防和治疗保护率。结果:黄芩提取物浓度为1.563mg·mL-1时,抑制病毒指数大于2,而柴胡组和配伍组的抑病毒指数均小于2;3组药物都具有不同程度的预防保护作用,而柴胡组和配伍组的预防保护作用尤为明显,其预防保护率明显高于黄芩组,有显著性差异(P<0.05);在病毒感染后,3组药物对细胞均具有不同程度的治疗保护作用,黄芩组和配伍组表现明显高于柴胡组,差异具有显著性(P<0.05)。结论:黄芩提取物具有明显的直接灭活病毒作用;3组药物都具有不同程度的预防保护作用和治疗保护作用,柴胡组和配伍组预防给药保护细胞作用明显;黄芩提取物和配伍提取物组的治疗保护作用明显;配伍组具有预防和治疗保护双重作用,但没有表现出配伍后作用增强的优势。Objective： To study the therapeutic effects of the skullcap,Bupleurum chinense and their compatible extract on cell infected by Coxsackievirus B3m（CVB3m） in vitro.Method： We used microcell culture method to observe the effect of the skullcap extract,the B.chinense extract and their compatible extract on antivirus and cell protection.We detected the restrained indexes of the three kinds of drugs on CVB3m and the cell protection rate by the three kinds of drugs when the cell was infected by CVB3m.Result： The skullcap extract could directly kill CVB3m in vitro when its concentration was 1.563 g·L-1,but the B.chinense extract and their compatible extract did not show this effect.The three kinds of drugs could protect cell infected by CVB3m.The effect of the B.group and the compatibility group was better than the skullcap groups when the drugs were administered in advance（P 0.05）.The effect of the skullcap group and the compatibility group were better than the B.chinense group when the drugs were administered after infection（P 0.05）.Conclusion： The three kinds of drugs can inhibit replication of CVB3m and protect cells from CVB3m.The effect of inhibiting virus of the Skullcap extract was better than the others.The effect of protecting cell of the B.chinense extract and the compatibility group were better than the Skullcap group.

关 键 词：黄芩 柴胡 中药提取 柯萨奇B3M病毒 抗病毒 体外 

分 类 号：R285.5[医药卫生—中药学]