NR2B-ERK-CREB信号途径参与了大鼠逃避性长期记忆  

Contribution of Nr2b-erk-creb signaling to the avoidance of rats' learning and memory ability

作　　者：陈旭红[1] 梁振江[2] 

机构地区：[1]深圳市第二人民医院中心实验室,518035 [2]深圳市儿童医院耳鼻咽喉科

出　　处：《齐齐哈尔医学院学报》2010年第13期2019-2021,共3页Journal of Qiqihar Medical University

摘　　要：目的明确NR2B-ERK-CREB途径是否参与了大鼠Y迷宫逃避性长期记忆。方法雄性SD大鼠45只(第一军医大学实验动物中心提供),共分为4组:ifenprodil腹腔注射组14只,DMSO腹腔注射组15只,此2组中分别有7只动物在Y-迷宫训练后立即处死,用来进行免疫组织化学染色;ifenprodil脑室注射组和DMSO脑室注射组各8只,分别在Y-迷宫记忆测试后立即脑室注射ifen-prodil(2.0μg/μl,注射量0.5μl/侧)或等量DMSO,并于记忆再巩固测试后立即断颈取脑,进行Westernblot蛋白免疫印迹检测。结果训练前15min腹腔注射ifenprodil组动物的Y迷宫学习成绩与DMSOip组相比,没有明显差异(P>0.05),但是在24h后测试动物的Y迷宫记忆,ifenprodilip组成绩差于DMSOip组成绩(P<0.05)。Y迷宫训练后ifenprodilip组各脑区pERK1/2和pCREB表达普遍下降,其中pERK1/2在海马、纹状体、杏仁核下降最为明显,pCREB在海马、纹状体、小脑最为明显,与DMSOip组相比差异显著(P<0.01)。第一次Y迷宫测试ifenprodilic组与DMSOic组成绩没有明显差异(P>0.05),测试后立即脑室注射并在术后6h测试动物对Y迷宫的记忆再巩固,ifenprodilic组成绩下降,与DMSOic组相比有明显差异(P<0.05),同时ifenprodilic组动物各被检测脑区pERK1/2和pCREB表达与DMSOic组相比均普遍下降。结论 NR2B-ERK-CREB途径参与了大鼠Y迷宫的习得、长期记忆形成、记忆的再巩固等过程,而且该途径对于长期记忆的形成和记忆提取是必要的。Objective To investigate whether NR2B-ERK-CREB signaling could contribute to the avoidance learning and memory of rat brain. Methods 45 adult male SD rats were divided into 4 groups： Ifenprodil peritoneal injection group（ifenprodil ip,n=14）, DMSO peritoneal injection group（DMSO ip,n=15）, ifenprodil cerebral ventricle injection group（ifenprodil ic,n=8）, DMSO cerebral ventricle injection group（DMSO ip,n=8）. Y-maze training and test were used as an learning and memory enhancing stimulus. Immunohistochemical and western blotting methods were used for detecting pERK1/2 and pCREB expression intensity of different brain regions. Results Compared with the DMSO ip group, the ifenprodil ip group showed no change on the Y-maze learning score （P〉0.05）, but its Y-maze memory score tested 24 after learning decreased （P〈0.05）. Ifenprodil peritoneal injection made brain pERK1/2 and pCREB expression decreased generally. In hippocampus, striatum, amygdale, cerebellum, these changes were more significant（P〈0.05）. Compared with the DMSO ic group, the reconsolidation of Y-maze memory tested 6 hours after ifenprodil injection was impaired in ifenprodil ic group（P〈0.05）. The OD value of pERK1/2 and pCREB positive bands in ifenprodil ic group attenuated generally. Conclusions NR2B was essential for the formation of long term memory, reconsolidation of Y-maze memory. The deactivation of NR2B by ifenprodil will impair these courses. Meanwhile, the deactivation of NR2B attenuates pERK1/2 and pCREB expression of learning and memory related regions after Y-maze learning and memory reconsolidation test.

关 键 词：NR2B PERK PCREB 学习与记忆 大鼠 

分 类 号：R338.64[医药卫生—人体生理学]