滇黄芩总黄酮急性毒性及抗实验性心律失常作用的研究  

Acute Toxicity of Total Flavonoid from Scutellaria amoena and its Effect on Experimental Arrhythmia

作　　者：何晓山[1] 代蓉[1] 陈秀红 侯安国[1] 陈小明[1] 

机构地区：[1]云南中医学院,昆明650500 [2]昆明市盘龙区人民医院,昆明650021

出　　处：《中国实验方剂学杂志》2010年第10期150-152,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：云南省教育厅自然科学研究重点资助项目(06Z333C)

摘　　要：目的:观察滇总黄芩黄酮提取物的小鼠急性毒性及对乌头碱诱发大鼠实验性心律失常的影响。方法:①以Bliss法测定滇黄芩总黄酮提取物的急性毒性;②通过测定大鼠心电图研究滇黄芩总黄酮的抗心律失常作用。结果:①滇黄芩总黄酮提取物对小鼠有一定急性毒性,但半数致死量(LD50,4.4388g·kg-1体重)与抗心律失常实验所采用的中剂量(20mg·kg-1体重)的剂量距离较远;②滇黄芩总黄酮提取物能明显增加乌头碱诱发大鼠室早(VP)、室颤(VF)和室性停搏(CA)所需的阈剂量;可剂量依赖性地缩短肾上腺素致麻醉豚鼠心律失常的持续时间。结论:滇黄芩总黄酮提取物对小鼠毒性小,但有明显的抗实验性心律失常作用。Objective：To study the acute toxicity of total flavonoid from Scutellaria amoena in mice and its effect on experimental arrhythmia induced by aconitine in rats. Method：The acute toxicity of the flavonoid was investigated by Blisss method. The anti-arrhythmia efftect of the flavonoid was determined by ECG of rats and of guinea pigs. Result：The flavonoid showed some toxicity on mice,but its median lethal dose （LD504. 438 8 g·kg ^-1） keeps away from effective median dose（20 mg·kg ^-1） of anti-arrhythmia activity experiment. It could obviously increase the threshold dose of ventricular premature beats（VP）,ventricular fibrillation（VF） and cardia ventricular arrest（CA） induced by aconitine in rats and shorten the duration of ventricular arrhythmia induced by adrenaline in guinea pigs with dose-dependent manner. Conclusion：The flavonoid from S. amoena has very little toxicity in mice,but obvious protective effect on arrhythmia models in animals.

关 键 词：滇黄芩总黄酮 急性毒性 实验性心律失常 

分 类 号：R285.5[医药卫生—中药学]