超声波法提取湿地松松针中莽草酸的研究  

Study on ultrasonic extraction technology of shikimic acid from Pinu elliottii Engelm

作　　者：谢济运[1,2] 陈小鹏[1] 陈芳[1] 

机构地区：[1]广西大学化学化工学院,南宁530004 [2]柳州师范高等专科学校,柳州545004

出　　处：《食品科技》2010年第8期258-262,共5页Food Science and Technology

基　　金：国家自然科学基金项目(30560119);广西自然科学基金项目(0575003);广西研究生教育创新计划项目(2006105930817M30);广西教育厅科学研究项目([2004]20);广西教育厅科学研究项目(200708LX180)

摘　　要：以乙醇为溶剂,利用超声辅助提取湿地松松针中的莽草酸,采用高效液相色谱法分析了提取物中莽草酸的含量。通过单因素实验分别考察了超声波功率、超声时间、乙醇浓度、料液比、超声温度和保持温度对莽草酸提取率的影响,筛选出主要因素和实验水平,由正交实验确定超声提取最佳工艺是:松针粒度40目、超声波功率250W、乙醇浓度65%、提取温度60℃、料液比1∶23和超声时间15min,莽草酸的提取率为1.73%。Ethanol used as solvent,shikimic acid in Pinu Elliottii engelm was extracted by ultrasonic wave,and then the content of shikimic acid was analyzed by high performance liquid chromatography.By the single factor experiment,the main influencing factors on extraction rate of shikimic acid by ultrasonic power,ultrasonic time,ethanol concentration,solid-liquid ratio,ultrasonic temperature and holding temperature were analyzed respectively.By the orthogonal test,the optimal extracting condition was confirmed as follows：the granularity of Pinu Elliottii Engelm was 40,ultrasonic power was 250 W,ethanol concentration was 65%,extraction temperature was 60 ℃,solid-liquid ratio was 1：23,extraction time was 15 min,extraction rate of shikimic acid was 1.73%.

关 键 词：湿地松针 莽草酸 超声波法 正交实验 

分 类 号：R284.2[医药卫生—中药学]