实验性糖尿病大鼠脑组织生化酶学及超微结构的研究  

BIOCHEMICO-ENZYMOLOGICAL AND ULTRASTRUCTURAL OF STUDY EXPERIMENTAL DIABETIC RAT BRAIN

作　　者：何涛[1] 李红辉[2] 吴明泸[1] 周志远[1] 刘友平[1] 杨烨[1] 

机构地区：[1]泸州医学院生物化学教研室,646000 [2]泸州医学院附属医院内分泌科,646000

出　　处：《泸州医学院学报》1999年第1期1-4,共4页Journal of Luzhou Medical College

摘　　要：目的：观察链脲佐菌素（SIZ）所致实验性糖尿病（DM）大鼠脑组织生化酶学及超微结构的改变。方法：用SIZ建造DM大鼠模型，6周后测定脑组织匀浆中ATP酸、乳酸脱氢酶（LDH）和乙酰胆碱酯酶（AchE）活性，并取脑组织作超微结构的观察。结果：实验性DM大鼠脑组织中Na+，K+－ATPase、Ca2+－ATPase、Mg2+－ATPase及AchE活性均显著降低（P＜0．01），而LDH活性却明显升高（P＜0．05），总抗氧化能力明显减低（P＜0．001））；电镜下可见神经元变性、坏死，线粒体肿胀、嵴断裂，细胞核空化消失，神经纤维变性。结论：在DM状态下，脑组织细胞的结构和功能均受到一定的损害。Objective: To observe the effects of experimental diabetes mellitus(DM) on the brain ofrat. Methods :The DM medel was set up with streptozotocin in rat . Six weeks later , the activies of ATPase 、lactate dehydrogenase(LDH) and ac etylcholinesterase (AchE ) in brain homgenates were meased. The brai n ti ssue was observed under elec -be nicroscope. Redults: It was shown that there were significan decare in activities of brainNa+, K+ - ATPase、Ca2+ - ATPase、Mg2+ - ATPase and AchE (P < 0.01 ), whereas of the achvity LDH was increased in bndn homoge-naes(P < 0.05). The total ability of anh - oxidaion in serum and bmin decnd ededly(P < 0. 001). Electron mi-cmecopy showed that there were denaturation and necrosis of neuron , swelling of mitochondria , the breaking of crista anddenaturation of neurofiber in brain of DM rat . Conclusions : The study indicate that the induchon of DM resulted in ab-normal metabolism and ultrastructure of brain cells , which mitht get involved in neuropathophysiology of diabetes .

关 键 词：糖尿病 脑组织 生化酶学 超微结构 实验研究 

分 类 号：R587.1[医药卫生—内分泌]