API_(0134)对凝血酶作用下血小板环核苷酸和胞浆游离钙离子浓度的影响  

Effects of API 0134 on the Thrombin induced Platelet Cyclic Nucleotide and Cytoplasmic Free Ca 2+

作　　者：李树生[1] 赵华月[1] 郭志凌[1] 

机构地区：[1]同济医科大学附属同济医院

出　　处：《同济医科大学学报》1999年第2期141-142,共2页Acta Universitatis Medicinae Tongji

基　　金：卫生部默沙东科研基金

摘　　要：为观察穿心莲有效成分（ＡＰＩ０１３４）对凝血酶作用下血小板活化的抑制作用，将家兔２４只随机分为ＡＰＩ０１３４（Ａ）组（ｎ＝９），噻氯匹定（Ｔ）组（ｎ＝８）和对照组（ｎ＝７），治疗１周，然后检测凝血酶作用下血小板内ｃＡＭＰ、ｃＧＭＰ和胞浆游离钙离子浓度，以及血小板超微结构。结果表明ｃＡＭＰ水平在Ａ、Ｔ两组显著高于Ｃ组（Ｐ＜０．０５），而胞浆游离钙离子浓度明显低于Ｃ组（Ｐ＜０．０１），超微结构表明Ａ组和Ｔ组血小板脱颗粒现象较Ｃ组明显减轻。在Ａ和Ｔ组之间各项参数均无统计学差异。In order to observe the inhibitory effect of Andrographis Paniculata Isolate 0134(API 0134 ,API) on the anti thrombin induced platelet activation, 24 domestic rabbits were randomly divided into API pretreated group ( n= 9), ticlopidine (T) pretreated group ( n= 8), and control (C) group ( n= 7). The rabbits received the treatment for 1 week. After induction with thrombin following parameters were measured: cAMP, cGMP and concentration of cytoplasmic free Ca 2+ . Ultrastructural changes of platelets were also examined. cAMP level was markedly higher in API and T groups than in C group ( P <0.05), while the concentration of free Ca 2+ in both API and T groups was markedly lower than in C group ( P <0.01). The ultrastructural changes of platelets showed the degranulation in API and T groups was much milder than in C group. API may inhibit thrombin induced activation of platelets.

关 键 词：API0134 环磷酸腺苷 钙离子 凝血酶 血小板活化 

分 类 号：R973[医药卫生—药品] Q582[医药卫生—药学]