四君子汤对脾虚大鼠胃肠运动功能和胃肠激素影响  

Effects of Sijunzi decoction on the gastrointestinal peristalsis and hormone secretion in spleen-insufficiency rats

作　　者：甘静宜[1] 程飞[1] 巩忠福[2] 程桂林[3] 朱晓宇[1] 王宁[3] 许剑琴[1,4] 刘凤华[3,5] 

机构地区：[1]中国农业大学动物医学院中兽医实验室,北京100081 [2]中国兽医药品监察所 [3]北京农学院动物科技系 [4]农业部兽用化药和中草药创制与评价重点开放实验室 [5]兽医学(中医药)北京市重点实验室

出　　处：《中兽医医药杂志》2010年第4期9-12,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然科学基金项目"胃肠激素与脾失健运相关性及四君子汤调控机理研究"(No.30771591);国家"十一五"科技支撑计划重点项目"中兽药现代化技术研究与开发"(2008BADB4B01;2008BADB4B07);"安全环保型中兽药的研制及应用"(2006BQD31B05-2)

摘　　要：为研究四君子汤对脾虚大鼠胃肠运动和胃肠激素含量影响,用营养性半固体糊灌胃法测定大鼠胃排空率和肠推进率,采集胃窦基础电位信号,ELISA法测定胃肠组织中CCK、MTL蛋白水平。结果显示,与对照组比,脾虚组胃排空率下降,肠推进率上升,胃电振幅和频率均降低;胃窦内CCK明显升高(P<0.05),十二指肠和空肠内CCK显著降低(P<0.05);MTL在脾虚大鼠消化道内呈整体降低趋势,仅在空肠内极显著降低(P<0.01)。与脾虚组比,治疗组胃排空率升高,肠推进率下降,胃电振幅和频率均回升;胃窦内CCK明显降低(P<0.05),十二指肠和空肠内CCK显著升高(P<0.05);消化道内MTL含量整体回升。由此可见,四君子汤可以平衡紊乱的胃肌电活动,调节脾虚大鼠的胃肠运动,调节消化道内局部CCK和MTL含量。Objective：To study the effects of Sijunzi decoction on the gastrointestinal peristalsis and hormone secretion in spleen-insufficiency rats.Methods：Stomach evacuation rates and intestine pushing rates were determined by gastric administration with nutritional semisolid.Basal electric potential of Gastric antrum was measured by gastro-electromyogram.The levels of CCK and MTL in gastrointestinal tract were determined by ELISA.Results：Compared with the control,the indexes of spleen-insufficiency group,such as stomach evacuation rates,swing and frequency of gastro-electricity were decreased,the levels of CCK in duodenum and jejunum were significantly decreased （P〈0.05）,the level of MTL in alimentary tract was decreased,especially in jejunum （P〈0.01）;on the contrary,intestine pushing rates were increased,and the level of CCK in gastric antrum was significantly increased （P〈0.05）.Compared with the spleen-insufficiency group,the indexes of treated group,such as stomach evacuation rates,swing and frequency of gastro-electricity were increased,the levels of CCK in duodenum and jejunum were significantly increased （P〈0.05）,the level of MTL in alimentary tract was increased;on the contrary,intestine pushing rates were decreased,and the level of CCK in gastric antrum was significantly decreased （P〈0.05）.Conclusion：Sijunzi decoction can regulate the gastrointestinal peristalsis and contents of CCK and MTL in alimentary tract in spleen-insufficiency rats.

关 键 词：脾虚 四君子汤 胃肠运动 胃肠激素 

分 类 号：S853.74[农业科学—临床兽医学]