1,4-二羟基蒽醌及其Y^(3+)配位聚合物与DNA相互作用研究  

Interaction of 1,4-Dihydroxyanthraquinone and Its Yttrium Coordination Polymer with DNA

作　　者：殷卫峰[1] 欧植泽[1] 高云燕[1] 郝平[1] 郭创龙[1] 王中丽[1] 

机构地区：[1]西北工业大学理学院应用化学系,西安710072

出　　处：《化学学报》2010年第14期1343-1348,共6页Acta Chimica Sinica

基　　金：教育部留学回国人员科研启动基金(NosN9YK0003;N9YK0005);西北工业大学基础研究基金(NoJC200822);西北工业大学翱翔之星(NoR0120)资助项目

摘　　要：利用紫外-可见吸收光谱(UV-Vis)、核磁共振(1HNMR)、红外光谱(FTIR)研究了1,4-二羟基蒽醌(DHA)与Y3+的配位作用,结果表明Y3+与DHA能形成物质的量之比为1:1的一维链状配位聚合物Y-DHA.与DHA相比,Y-DHA在可见光区的吸收大幅增强,同时具有良好的水溶性.紫外-可见吸收光谱、荧光光谱及DNA熔链温度实验研究结果表明,Y-DHA与CTDNA可通过静电作用和沟槽结合的方式结合,而DHA与CTDNA的作用方式主要为沟槽结合.循环伏安法表明,Y-DHA的还原电位(-0.324VvsSCE)要高于DHA的还原电位(-0.387VvsSCE).无氧条件下,Y-DHA光损伤DNA的能力要明显高于DHA.The interaction of 1,4-dihydroxyanthraquinone（DHA） with Y^3＋ was studied by UV-Vis,^1H NMR,and FTIR.Y^3＋ and DHA can form one-dimensional chain coordination polymer Y-DHA with a 1：1 stoichiometry.Y-DHA possesses better water solubility and shows stronger absorption in visible region than DHA.The interaction of Y-DHA and DHA with CT DNA was studied by UV-Vis,fluorescence spectra,and DNA melting temperature experiments.The results indicate that the groove binding and electrostatic interaction are the major modes for the association of Y-DHA with CT DNA,while groove binding is the mainly mode for the interaction of DHA with CT DNA.Voltammetry studies demonstrate that the reduction potential of Y-DHA（-0.324 V vs SCE） is more positive than that of DHA（-0.387 V vs SCE）.Under anaerobic conditions,Y-DHA shows stronger DNA photocleavage ability than DHA.

关 键 词：蒽醌 配位聚合物 DNA光损伤 光诱导电子转移 

分 类 号：TQ460.4[化学工程—制药化工]