用二维核磁共振研究[Co(phen)_2(HNAIP)]Cl_3配合物与错配及正常寡聚核苷酸的作用  

Study on the Interaction between Mismatched Oligonucleotide and Normal Oligonucleotide with [Co(phen)_2(HNAIP)]Cl_3 by 2D NMR

作　　者：席小莉[1] 杨曼曼[2] 陈绘丽[1] 杨频[1] 

机构地区：[1]山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,太原030006 [2]山西大学化学化工学院,太原030006

出　　处：《化学学报》2010年第14期1415-1420,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No20601018)资助项目

摘　　要：为了开发能识别正常与错配核酸的探针,首次合成了[Co(phen)2(HNAIP)]Cl3金属配合物,并利用二维核磁研究了此配合物与错配d(CCGAATGAGG)2及正常d(CCTAATTAGG)2寡聚核苷酸的相互作用,首次发现,[Co(phen)2(HNAIP)]Cl3是从"大沟"插入到错配碱基对G3A4和G7A8之间,能够很好地识别G:A错配;而[Co(phen)2(HNAIP)]Cl3与正常的寡聚核苷酸的作用,则是从小沟插入,即[Co(phen)2(HNAIP)]Cl3与错配和正常寡聚核苷酸在不同位置及不同沟槽存在明显的插入式结合、并且有明显的差异,这就有可能被用来作为识别正常与错配寡聚核苷酸的探针.In order to develop the probe that can identify mismatched oligonucleotide and normal oligonucleotide,we synthesized [Co（phen）2（HNAIP）]Cl3 metal complex firstly,and studied the interaction between mismatched oligonucleotide d（CCGAATGAGG）2 and normal oligonucleotide d（CCTAATTAGG）2 with [Co（phen）2（HNAIP）]Cl3 by 2D NMR.The results indicate that the complex binds the mismatched oligonucleotide by intercalation with the HNAIP ligand selectively inserted from the major groove between the stacked bases in the G3A4 and G7A8 region.So,it can identify G：A mismatch.At the same time,the complex binds the normal oligonucleotide by intercalation with the HNAIP ligand from the minor groove between the stacked bases in the terminal region.Because [Co（phen）2（HNAIP）]Cl3 can interact with mismatched oligonucleotide and normal oligonucleotide on different location and by different style,it can be used as a probe to identify mismatched oligonucleotide and normal oligonucleotide.

关 键 词：二维NOSY谱 寡聚核苷酸 DNA 

分 类 号：R341[医药卫生—基础医学]