应用荧光染色法检测寄主昆虫体表病原真菌孢子及其附着孢  

Test and Determination of Entomopathogenic Fungal Spore and Appressorium on Its Host with Calcofluor White M2R

作　　者：曹月青[1] 朱祥先[1] 

机构地区：[1]重庆大学生物工程学院基因工程中心重庆市杀虫真菌生物农药工程中心

出　　处：《微生物学杂志》2010年第3期91-92,共2页Journal of Microbiology

基　　金：国家自然科学基金(30800736);教育部博士点新教师基金(20070611043);重庆大学“211工程”三期建设研究生开放实验室项目(S-09014)

摘　　要：采用蝗虫翅膀作为侵染组织,探讨了荧光染色剂Calcofluor White M2R在观测寄主体表绿僵菌孢子及其附着孢形成中的应用。结果表明,在荧光显微镜下,清晰可见蝗虫翅膀上发蓝色荧光的绿僵菌孢子、芽管及附着孢,而蝗虫翅膀未被染色,避免了干扰观察目标物。该方法可以准确观察病原真菌孢子在昆虫体表组织的萌发及附着孢形成。Locust wings were used as infection tissues in this study, to investigate the application of fluorescent stain Calcofluor White M2R for observation of Metarhizium anisopliae spores and the formation of its appressorium. The results showed that under fluorescence microscopy blue fluorescence could be seen clearly, showing the detailed shapes and characteristics of spores, germ tubes, and appressorium of Metarhizium anisopliae on the wing’s surface of locust （Migratoria manilensis）. Since the locust wing itself was not stained and it became dark background under fluorescence microscopy, which eliminated possible background that interfering the observation of the object. This method could be accurately used for the observation of the germination of pathogenic fungal spores and the formation of appressorium of entomopathogenic fungi on their surface of the host insects.

关 键 词：昆虫病原真菌 绿僵菌 附着孢 CALCOFLUOR White M2R 荧光染色 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]